Results 111 to 120 of about 559,125 (302)
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Applying the possibilistic C-means algorithm in kernel-induced spaces [PDF]
, 2010 In this paper, we study a kernel extension of the classic possibilistic c-means. In the proposed extension, we implicitly map input patterns into a possibly high-dimensional space by means of positive semidefinite kernels. In this new space, we model the
Masulli, F. +5 more
core +1 more source
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Improved K-means algorithm for manufacturing process anomaly detection and recognition
, 2006 Anomaly detection and recognition are of prime importance in process industries. Faults are usually rare, and, therefore, predicting them is difficult .
Shi HB(史海波) +2 more
core
FEBS Open Bio, EarlyView.In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes +32 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
On Sampling Based Algorithms for k-Means.
, 2020 We generalise the results of Bhattacharya et al. [Bhattacharya et al., 2018] for the list-k-means problem defined as - for a (unknown) partition X₁, ..., X_k of the dataset X ⊆ ℝ^d, find a list of k-center-sets (each element in the list is a set of k centers) such that at least one of k-center-sets {c₁, ..., c_k} in the list gives an (1+ε ...
Anup Bhattacharya +3 more
openaire +3 more sources
FEBS Open Bio, EarlyView.ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Enhanced Polycystic Ovary Syndrome Diagnosis Model Leveraging a K-means Based Genetic Algorithm and Ensemble Approach [PDF]
Polycystic Ovary Syndrome (PCOS) is a prevalent hormonal disorder affecting women in their childbearing years. Detecting PCOS early is crucial for preserving fertility in young women and preventing long-term health complications like hypertension, heart ...
Sahi, Aqeel +4 more
core +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source