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Determination of hydride transfer stereospecificity of NADH-dependent alcohol-aldehyde/ketone oxidoreductase from Sulfolobus solfataricus

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1990
This paper describes the determination of stereospecificity of hydride transfer reaction of an alcohol dehydrogenase isolated from the archaebacterium Sulfolobus solfataricus. The 1H-NMR and EI-MS data indicate that the enzyme transfers the pro-R hydrogen from coenzyme to substrate and is therefore an A-specific dehydrogenase.
A Trincone   +5 more
openaire   +4 more sources

An alternative bioreactor concept for application of an isolated oxidoreductase for asymmetric ketone reduction

Tetrahedron, 2004
In this paper an isolated NADH dependent ketone reductase has been used to synthesise (S)-6-bromo-β-tetralol from 6-bromo-β-tetralone, together with commercially available formate dehydrogenase (FDH) as a recycle enzyme to produce preparative quantities of the product.
Vicky J. Shorrock   +2 more
openaire   +1 more source

[NAD(P)H-dependent oxidoreductases for synthesis of chiral amines by asymmetric reductive amination of ketones].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2020
Asymmetric reductive amination is one of the most important reactions in the preparation of chiral amine pharmaceutical intermediates. Different kinds of enzymes have been applied to the synthesis of chiral amines Among them, the development of reductive amination reactions catalyzed by NAD(P)H-dependent oxidoreductases is attractive, as it would ...
Feng, Cheng   +3 more
openaire   +1 more source

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