Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins
We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó +7 more
wiley +1 more source
Report on the 2nd MObility for Vesicle research in Europe (MOVE) symposium—2024
The 2nd MObility for Vesicle research in Europe (MOVE) Symposium in Belgrade brought over 280 attendees from 28 countries to advance extracellular vesicle (EV) research. Featuring keynotes, presentations, and industry sessions, it covered EV biogenesis, biomarkers, therapies, and manufacturing.
Dorival Mendes Rodrigues‐Junior +5 more
wiley +1 more source
A chromosome-level genome assembly of the Chinese herbal medicine Chelidonium majus. [PDF]
Bu X +12 more
europepmc +1 more source
Analysis of the key factors for small and medium-sized enterprises growth using principal component analysis [PDF]
Samson O. Ongbali +4 more
openalex +1 more source
Here, we introduced an intermittent electrical stimulation protocol mimicking the episodic nature of real‐life exercise in vitro by alternating low‐ and high‐frequency stimulation. In comparison with widely used continuous stimulation, it enhanced the rate of glucose and fatty acid oxidation, but not the myokine release.
Klára Gabrišová +11 more
wiley +1 more source
Dual process impairments in reinforcement learning and working memory systems underlie learning deficits in physiological anxiety. [PDF]
Senta JD, Bishop SJ, Collins AGE.
europepmc +1 more source
The impact of Hnrnpl deficiency on transcriptional patterns of developing muscle cells
We performed nanopore whole‐transcriptome sequencing comparing RNA from Hnrnpl‐knockdown versus control C2C12 myoblasts to investigate the contributions of Hnrnpl to muscle development. Our results indicate that Hnrnpl regulates the expression of genes involved with Notch signaling and skeletal muscle, particularly splicing patterns of specific muscle ...
Hannah R. Littel +8 more
wiley +1 more source
Extremely poleward shift of Antarctic Circumpolar Current by eccentricity during the Last Interglacial. [PDF]
Lu L +25 more
europepmc +1 more source
Sarcosine oxidase from Bacillus sp. (SoxB) recognizes l‐proline (l‐Pro), d‐proline (d‐Pro), and l‐thioproline (l‐Tpr) as minor substrates. In this study, we measured their absorption spectra, determined the crystal structures of their enzyme complexes, and performed site‐directed mutagenesis of key residues.
Yuqi Zhang +5 more
wiley +1 more source
Low-Input Assay for Transposase-Accessible Chromatin Identifies Epigenetic Signatures of Liver Group 1 Innate Lymphoid Cells. [PDF]
Schmid K, Schenk RP, Wiedemann GM.
europepmc +1 more source

