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[18] Metabolism of isolated kidney tubule segments

1990
Publisher Summary This chapter presents a short review on the isolation and analysis of defined nephron segments, giving some examples of the determination of enzyme activities and metabolic pathways in them. The advantages and disadvantages of the different techniques are also discussed. To study metabolite concentrations, enzyme activities, hormone
Walter G. Guder, Gabriele Wirthensohn
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The electrical profile of the distal tubule in triturus kidney

Pfl�gers Archiv European Journal of Physiology, 1982
The transepithelial potential difference (VTE) and transepithelial resistance (RTE) were determined along the length of the distal tubule of the amphibian Triturus alpestris. The site of impalements was determined at the end of each experiment by latex injection and microdissection.
J. Teulon, T. Anagnostopoulos
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Inhibition of kidney tubule induction by charged polymers

Cell Differentiation, 1978
Charged polymers, known to alter cell surface characteristics, reversibly inhibit the intercellular communication required in kidney tubule induction. This effect seems to be a function of their charge density. It is suggested that this effect is due to the formation of a molecular barrier between the interacting cell surfaces.
Stig Nordling   +2 more
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A Kallikrein-Specific Inhibitor in Rat Kidney Tubules

Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1976
A kallikrein inhibitor was found in tubules of the rat kidney and purified by chromatography on Sephadex G-100. The molecular weight of the inhibitor, estimated by gel filtration and dodecylsulfate electrophoresis, is about 4700. It inhibits the following kallikreins: porcine submanidbular and pancreatic kallikrein, rat kidney and urine kallikrein, and
Reinhard Geiger, Klaus Mann
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Isolated rat kidney tubules as a screening system for arsenic antidotes.

Toxicology and Applied Pharmacology, 1993
Gluconeogenesis is one of the metabolic pathways severely affected in acute arsenic poisoning. We have studied gluconeogenesis in isolated kidney tubules of male Sprague-Dawley rats to screen various sulfur compounds for antidotal properties against ...
H. Mückter   +6 more
semanticscholar   +1 more source

Single-cell analysis identifies the interaction of altered renal tubules with basophils orchestrating kidney fibrosis

Nature Immunology, 2022
T. Doke   +18 more
semanticscholar   +1 more source

Uptake of 125I-labelled prolactin by bullfrog kidney tubules: an autoradiographic study.

Journal of Endocrinology, 1982
The uptake of 125I-labelled ovine prolactin in the bullfrog kidney was studied by autoradiography. Five minutes after the intracardiac injection of 125I-labelled prolactin, no labelling was detected in the kidneys of premetamorphic tadpoles or in animals
O. Gona
semanticscholar   +1 more source

A road to kidney tubules via the Wnt pathway

Pediatric Nephrology, 2000
Classical in vitro studies indicate that tubule induction in the kidney mesenchyme is mediated by cell-cell contacts between the inducer tissue and the metanephric mesenchyme. Induction is completed within the first 24 h, after which tubules will form because of stimulated cell proliferation, migration, and cell adhesion.
Marika S. Uusitalo, Seppo Vainio
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SEM of the proximal tubule of the adult rabbit kidney

The Anatomical Record, 1978
AbstractThe present study utilizes the scanning electron microscope (SEM) to reveal the surface morphology of proximal tubular cells and the parietal cells of Bowman's capsule of the adult rabbit nephron. To facilitate the examination of the basal surface of these cells, proximal tubules were dissected free and treated with collagenase to remove the ...
Don A. Hay   +2 more
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Phenylalanine hydroxylation in isolated rat kidney tubules

International Journal of Biochemistry, 1990
1. Phenylalanine hydroxylation has been demonstrated to occur in isolated rat kidney tubules under physiological conditions. 2. The hydroxylation flux response is hyperbolic with apparent Km and Vmax values of ca 85 microM phenylalanine and 49 nmol tyrosine formed/mg dry wt per hr respectively. 3.
Anne K. Green   +3 more
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