Results 161 to 170 of about 2,344,694 (261)

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Amputation kit knife or scalpel

This American Civil War-era field amputation kit is housed in a rosewood case with a brass plate on the lid stamped with the name “Elmer David Charland M.D.” The box has brass corner plates and a brass key lock with key.
H. G. Kern (Philadelphia, Pa.)
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The Clinical and Molecular Characteristics of Systemic Mastocytosis with Associated Non-Mast Cell Myeloid Neoplasm. [PDF]

J Clin Med
Seth N, Deb PQ.
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Dedifferentiated Gastric Gastrointestinal Stromal Tumor with Rapid Growth Mimicking Other Malignancies: A Case Report. [PDF]

Surg Case Rep
Iijima K, Takahashi T, Kurokawa Y, Saito T, Hagi T, Nakai S, Momose K, Yamashita K, Tanaka K, Makino T, Nakajima K, Genko Y, Kurashige M, Morii E, Eguchi H, Doki Y.   +15 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

KIT mutation and AHN-associated oncogenic profiles in systemic mastocytosis with an associated hematological neoplasm. [PDF]

Blood Adv
Navarro-Navarro P, Fernández-Giménez CM, García-Montero AC, Álvarez-Twose I, Sánchez-Muñoz L, Henriques A, Pérez-Pons A, González-Tablas M, Teodosio C, González-González M, Matarraz S, López A, Barcena P, Colado E, Esteve J, Senent ML, González-López Ó, Mollejo M, Morales-Cabeza C, Jara-Acevedo M, Orfao A.   +20 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Development of the cf-MMSP Assay for Enhanced Analytical Sensitivity of Methylated DNA in Breast Cancer Liquid Biopsy. [PDF]

Cancer Epidemiol Biomarkers Prev
Zhang LQ, Xia W, Yu G, Saravanan R, Fackler MJ, Pleas M, Cope L, Stearns V, Lehman J, Wolff AC, Sukumar S.   +10 more
europepmc   +1 more source