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Behaviour of the yeast Kluyveromyces marxianus var. marxianus during its autolysis

Antonie van Leeuwenhoek, 1996
The lactic yeast Kluyveromyces marxianus var.marxianus (formerly K. fragilis) autolyzates at faster rate than Saccharomyces cerevisiae. During K. marxianus autolysis, quite similar release kinetics were observed for intracellular space markers (potassium ions, nucleotides), cell-wall components (polysaccharides, N-acetyl-D-Glucosamine) and non specific
Amrane, A., Prigent, Yvon
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Characterizing yeast promoters used in Kluyveromyces marxianus

World Journal of Microbiology and Biotechnology, 2015
Fermentation at higher temperatures can potentially reduce the cooling cost in large-scale fermentation and reduce the contamination risk. Thus, the thermotolerant yeast, Kluyveromyces marxianus, which can grow and ferment at elevated temperatures, is a promising biotechnological tool for future applications. However, the promoters used in K. marxianus
Chun, Yang   +5 more
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Kluyveromyces marxianus: a potential source of diacetyl reductase

World Journal of Microbiology & Biotechnology, 1994
Kluyveromyces marxianus had a higher specific activity of diacetyl reductase (EC 1.1.1.5) than all other organisms previously reported. The enzyme was NADH-dependent and irreversibly catalysed the conversion of diacetyl to acetoin with an optimum pH of 7.0. It was stable at 40°C but lost 50% of its activity at 50°C in 30 min.
J G, Schwarz, Y D, Hang
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γ‐aminobutyric acid production by Kluyveromyces marxianus strains

Journal of Applied Microbiology, 2020
Kluyveromyces marxianus dairy strains were tested for γ-aminobutyric acid (GABA) production. The genes involved in GABA catabolism (UGA1 and UGA2) and anabolism (GAD1) were found in K. marxianus genome. Their relative expression was evaluated with primer designed ad hoc.Strains were grouped on the basis of GAD1 gene sequence. Representative strains for
Perpetuini G.   +4 more
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Structure of the ABF1-homologue from Kluyveromyces marxianus

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1993
By transformation of a Saccharomyces cerevisiae mutant strain conditionally expressing the ABF1-gene, a Kluyveromyces marxianus DNA fragment carrying the gene encoding the ABF1-homologue of this yeast strain (KmABF1) was selected. Comparison of the sequence of the KmABF1 gene with that encoding Saccharomyces cerevisiae ABF1 and the previously isolated ...
E H, Oberyé   +3 more
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Cloning and characterization of Kluyveromyces marxianus Hog1 gene

Biotechnology Letters, 2010
The mitogen-activated protein kinase Hog1 gene (Kmhog1) was isolated from Kluyveromyces marxianus strain NBRC 1777 by degenerate PCR and genome walking, and then disrupted to construct a mutant strain hog1∆. The mutant was now more sensitive to acetic acid and its growth was nearly completely inhibited by 0.5 M NaCl (97%) and 10 mM H(2)O(2) (93%) as ...
Jiangchao, Qian   +4 more
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Metabolic physiology of aroma‐producing Kluyveromyces marxianus

Yeast, 2002
AbstractKluyveromyces marxianus has a high potential for industrial production of aroma compounds, such as 2‐phenylethanol, which is derived in a bioconversion from L‐phenylalanine. In the present work the product yield of K. marxianus in batch cultivation was estimated as 0.65 mol 2‐phenylethanol/mol L‐phenylalanine and thus significantly below the ...
Christoph, Wittmann   +2 more
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Iminodiacetate and Nitrilotriacetate Degradation by Kluyveromyces marxianus IMB3

Biochemical and Biophysical Research Communications, 2002
The thermotolerant yeast Kluyveromyces marxianus IMB3 was capable of utilising either iminodiacetate or nitrilotriacetate as a sole source of nitrogen for growth. Cell extracts contained iminodiacetate dehydrogenase and nitrilotriacetate monooxygenase activities, suggesting the presence in the yeast of orthologues of these bacterial enzymes.
Nigel G, Ternan, Geoffrey, McMullan
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Applications of Kluyveromyces marxianus in Biotechnology

2017
Kluyveromyces marxianus is a member of the Saccharomycetales yeast order and is used for a variety of commercial applications, most notably production of ethanol from food waste streams. Traits such as rapid growth rates, lactose utilization, good tolerance to inhibitory molecules and thermotolerance facilitate these applications. K.
Javier A. Varela   +4 more
openaire   +1 more source

Metal-enzyme interactions in ADH1 from Kluyveromyces marxianus

International Journal of Biochemistry, 1993
1. 1. Among all metals tested (Cu2+, Ni2+, Co2+, Mn2+, Zn2+) only Cu2+ and Ni2+ can exert an inhibitory effect on catalysis. 2. 2. The effect of divalent cations (copper and nickel) on alcohol dehydrogenase 1 (ADH1) from Kluyveromyces marxianus is a mixed type inhibition. 3. 3.
GASTALDI, Daniela   +3 more
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