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Label-Free Isolation of Exosomes Using Microfluidic Technologies.
ACS Nano, 2021Exosomes are cell-derived structures packaged with lipids, proteins, and nucleic acids. They exist in diverse bodily fluids and are involved in physiological and pathological processes.
Sara Hassanpour Tamrin +2 more
semanticscholar +1 more source
Journal of Proteome Research, 2020
Relative label-free quantification (LFQ) of shotgun proteomics data using precursor (MS1) signal intensities is one of the most commonly used applications to comprehensively and globally quantify proteins across biological samples and conditions.
Anup D. Shah +4 more
semanticscholar +1 more source
Relative label-free quantification (LFQ) of shotgun proteomics data using precursor (MS1) signal intensities is one of the most commonly used applications to comprehensively and globally quantify proteins across biological samples and conditions.
Anup D. Shah +4 more
semanticscholar +1 more source
2021
In this chapter, we focus on label-free biosensors. First, we look at sensors with optical detection. In this context, the Mach–Zehnder interferometer and the surface plasmon resonance offer the possibility to limit the optical examination to shallow surface layers. The second group are the gravimetric biosensors.
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In this chapter, we focus on label-free biosensors. First, we look at sensors with optical detection. In this context, the Mach–Zehnder interferometer and the surface plasmon resonance offer the possibility to limit the optical examination to shallow surface layers. The second group are the gravimetric biosensors.
openaire +1 more source
Label-Free Microelectronic PCR Quantification
Analytical Chemistry, 2006We present a robust and simple method for direct, label-free PCR product quantification using an integrated microelectronic sensor. The field-effect sensor can sequentially detect the intrinsic charge of multiple unprocessed PCR products and does not require sample processing or additional reagents in the PCR mixture.
Chih-Sheng Johnson, Hou +5 more
openaire +2 more sources
2009
Label-free biosensors are devices that use biological or chemical receptors to detect analytes (molecules) in a sample. They give detailed information on the selectivity, affinity, and, in many cases, also the binding kinetics and thermodynamics of an interaction. Although they can be powerful tools in the hands of a skilled user, there is often a lack
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Label-free biosensors are devices that use biological or chemical receptors to detect analytes (molecules) in a sample. They give detailed information on the selectivity, affinity, and, in many cases, also the binding kinetics and thermodynamics of an interaction. Although they can be powerful tools in the hands of a skilled user, there is often a lack
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Nature Methods, 2009
New methods to coax signals from unlabeled biological molecules may finally fulfill the promise of practical label-free microscopy with molecular specificity.
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New methods to coax signals from unlabeled biological molecules may finally fulfill the promise of practical label-free microscopy with molecular specificity.
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Label-Free Functional Selectivity Assays
2015G protein-coupled receptors (GPCRs) represent the largest class of drug targets. Ligand-directed functional selectivity or biased agonism opens new possibility for discovering GPCR drugs with better efficacy and safety profiles. However, quantification of ligand bias is challenging.
Ann M, Ferrie +3 more
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Label-Free Proteomics of Serum
2008In this chapter we describe a method to analyze human serum with the goal of discovering disease-related changes in the serum proteome. The methodology is based on the removal of the six most abundant serum proteins by immunoaffinity chromatography. This step is followed by trypsin digestion and reversed-phase high-performance liquid chromatography ...
Govorukhina, N.I +2 more
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Label-Free Resistive-Pulse Cytometry
2011Numerous methods have recently been developed to characterize cells for size, shape, and specific cell-surface markers. Most of these methods rely upon exogenous labeling of the cells and are better suited for large cell populations (>10,000). Here, we review a label-free method of characterizing and screening cells based on the Coulter-counter ...
M R, Chapman, L L, Sohn
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Label-free optical biosensing:
Chemical Society Reviews, 2023Label-free optical biosensing holds great promise for a variety of applications in biomedical diagnostics, environmental and food safety, and security. It is already used as a key tool in the investigation of biomolecular binding events and reaction constants in real time and offers further potential additional functionalities and low-cost designs ...
Kabashin, Andrei V +2 more
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