Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Appropriateness of Histopathology Specimen Containers and Completeness of Laboratory Request Forms in Uganda: A Case of Mbarara Regional Referral Hospital, South Western Uganda. [PDF]
Mitala Y +12 more
europepmc +1 more source
Dolores Archaeological Program Technical Reports, DAP-015: Laboratory Operations - 1978
Paul Farley
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Overview of molecular signatures of senescence and associated resources: pros and cons
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
wiley +1 more source
Reducing Photo-Oxidative Stress in IVF: A Retrospective Analysis of Cycles with Poor Blastocyst Development. [PDF]
Gödöny K +5 more
europepmc +1 more source
Integration of Inquiry Laboratory Activities in Archimedes’ Law Learning
Ivan Setia Arianto +2 more
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The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan +6 more
wiley +1 more source
Correction: Stroma-driven horizontal transfer of TCA-related proteins mediates metabolic plasticity and Imatinib resistance in chronic myeloid leukemia. [PDF]
Chroscicki P +15 more
europepmc +1 more source
Chemotherapies such as doxorubicin can have toxic effects on healthy cardiovascular/heart tissue. Following up on a doxorubicin toxicity study in mice without tumors where nitrate water was cardioprotective (lessened toxicity), this study with tumor‐bearing mice undergoing doxorubicin treatment showed no negative effect of nitrate and nitrite on drug ...
Rama D. Yammani +7 more
wiley +1 more source

