Long-term continuous atmospheric CO2 measurements at Baring Head, New Zealand [PDF]
We present descriptions of the in situ instrumentation, calibration procedures, intercomparison efforts, and data filtering methods used in a 39-yr record of continuous atmospheric carbon dioxide (CO2) observations made at Baring Head, New Zealand ...
S. E. Nichol +6 more
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Bioconversion of vitamin D3 into calcitriol by Actinomyces hyovaginalis isolate CCASU- A11-2
Vitamin D3 is a fat-soluble prohormone that is activated inside the liver to produce 25-hydroxyvitamin D3 (calcidiol), and in the kidney to produce the fully active 1α, 25-dihydroxy vitamin D3 (calcitriol).
Ahmad M. Abbas +4 more
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A relaxed eddy accumulation flask sampling system for 14C-based partitioning of fossil and non-fossil CO2 fluxes [PDF]
A relaxed eddy accumulation (REA) system was developed and tested, enabling conditional sampling of air for subsequent 14CO2 analysis. This allows a 14C-based estimation of fossil fuel CO2 concentrations in the collected air samples and, thus, an ...
A.-K. Kunz +17 more
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Improving the automated calibration at Belle II [PDF]
In 2019 a Python plugin package, b2cal, based on the Apache Airflow workflow management platform was developed to automate the calibration at Belle II.
Pham Francis +2 more
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Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay
Background The split GFP assay is a well-known technology for activity-independent screening of target proteins. A superfolder GFP is split into two non-fluorescent parts, GFP11 which is fused to the target protein and GFP1-10.
Carolin Müller +3 more
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Protocol for generating reproducible miniaturized controlled midbrain organoids
Summary: Here, we present a protocol for generating miniaturized controlled midbrain organoids (MiCOs) of reproducible size and cellular composition, without a necrotic center. We describe steps for maintaining and passaging human pluripotent stem cells,
Muwan Chen +2 more
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The purpose of the present study was to harmonize the protocol of equilibrium solubility measurements for poorly water-soluble drugs to lower inter-laboratory variance.
Asami Ono +15 more
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Fluid Dynamics Appearing during Simulated Microgravity Using Random Positioning Machines. [PDF]
Random Positioning Machines (RPMs) are widely used as tools to simulate microgravity on ground. They consist of two gimbal mounted frames, which constantly rotate biological samples around two perpendicular axes and thus distribute the Earth's gravity ...
Simon L Wuest +3 more
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An Effective System to Produce Smoke Solutions from Dried Plant Tissue for Seed Germination Studies
Premise of the study: An efficient and inexpensive system was developed to produce smoke solutions from plant material to research the influence of water-soluble compounds from smoke on seed germination.
Janice Coons +4 more
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A simple method for isolation of rest of trypsinized stem cells with magnetic beads
Till now, there is no laboratory in the world, which makes thoughts that there may be still stem cells remaining in the flasks after the trypsin treatment.
Sudhir Bhatia
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