Results 51 to 60 of about 305,465 (335)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli

BioTechniques, 2008
We have developed an automated fermentation system for cost-efficient upscaling of protein expression in bacteria. The system, built for use by nonbiotechnologists, can be assembled mostly from standard laboratory equipment and allows a largely ...
Uwe Riek, Roland Tuerk, Theo Wallimann, Uwe Schlattner, Dietbert Neumann   +4 more
doaj   +1 more source

Direct in-vial collection for liquid-scintillation assay of carbon-14 and tritium [PDF]

, 1969
Dissolution of biological materials combines the simplicity of oxygen-flask combustion with the reproducibility and purity of the final product, and convenience of direct in-vial collection of the sample by the sealed-tube method. It assures quantitative
Huebner, L. G., Kisieleski, W. E.
core   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Significance of bioleaching method in dissolution of iron and in the quality improvement of non-metallics [PDF]

Acta Montanistica Slovaca, 2006
Simple laboratory bioleaching experiments for the iron removal with heterotrophic bacteria on natural raw materials were conducted to explore a simple cyclic operation for a potential use at the industrial scale.
Iveta Štyriaková, Igor Štyriak
doaj  

Differential Uptake of Gold Nanoparticles by 2 Species of Tadpole, the Wood Frog (Lithobates Sylvaticus) and the Bullfrog (Lithobates Catesbeianus)

, 2017
Engineered nanoparticles are aquatic contaminants of emerging concern that exert ecotoxicological effects on a wide variety of organisms. We exposed cetyltrimethylammonium bromide–capped spherical gold nanoparticles to wood frog and bullfrog tadpoles ...
Andresen, Kurt, Bury, Taylor B., Carfagno, Gerardo L.F., Fong, Peter P., Lee, Laura L., Lerner, Kevin T., Sitton, Andrea J., Thompson, Lucas B.   +7 more
core   +1 more source

Measurement of the Light Antiquark Flavor Asymmetry in the Nucleon Sea [PDF]

, 1998
A precise measurement of the ratio of Drell-Yan yields from an 800 GeV/c proton beam incident on hydrogen and deuterium targets is reported. Over 140,000 Drell-Yan muon pairs with dimuon mass M_{mu+ mu-} >= 4.5 GeV/c^2 were recorded. From these data, the
A. Baldit, A. D. Martin, A. Signal, A. Szczurek, A. W. Thomas, B. A. Mueller, B. K. Park, C. A. Gagliardi, C. N. Brown, D. A. Ross, D. D. Koetke, D. F. Geesaman, D. K. Wise, D. M. Alde, D. M. Kaplan, D. M. Lee, E. A. Hawker, E. J. Eichten, E. J. Eichten, E. M. Henley, G. Kyle, G. Moreno, G. Petitt, G. R. Young, G. T. Garvey, H. L. Lai, J. C. Peng, J. C. Webb, J. D. Bush, J. L. Willis, J. M. Moss, J. Selden, K. Gottfried, L. D. Isenhower, M. A. Vasiliev, M. Arneodo, M. E. Beddo, M. E. Sadler, M. J. Leitch, N. Makins, P. Amaudruz, P. E. Reimer, P. L. McGaughey, P. L. McGaughey, P. M. Nord, P. N. Kirk, P. W. Stankus, R. E. Tribble, R. S. Towell, S. B. Kaufman, S. D. Drell, S. D. Ellis, S. Kumano, S. Kumano, S. Kumano, T. A. Carey, T. C. Awes, T. H. Chang, T. N. Thompson, V. Papavassiliou, W-Y. P. Hwang, W. E. Cooper, W. E. Sondheim, W. M. Lee, X. C. He, Y. C. Wang, Z. F. Wang   +66 more
core   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Denitrification by rhizobia: A possible factor contributing to nitrogen losses from soils [PDF]

, 1980
The intensive pastoral farming system on which New Zealand animal production is based is almost completely dependent upon the rhizobium-legurne symbiosis for the fixed nitrogen required for pasture production.
Daniel, Roy M., Limmer, A.W., Steele, K.W.   +2 more
core   +1 more source

Cutaneous Melanoma Drives Metabolic Changes in the Aged Bone Marrow Immune Microenvironment

Aging and Cancer, EarlyView.
Melanoma, the deadliest form of skin cancer, increasingly affects older adults. Our study reveals that melanoma induces changes in iron and lipid levels in the bone marrow, impacting immune cell populations and increasing susceptibility to ferroptosis.
Alexis E. Carey, Murilo Ramos Rocha, Cheyenne Palm, Vania Wang, Denys Balandin, Ethan Black, Kevin Y. Zhang, Fan Huang, Daniel J. Zabransky, Yash Chhabra, Mareike Peters, Gabriel Ghiaur, Ashani Weeraratna   +12 more
wiley   +1 more source