Results 81 to 90 of about 4,804,166 (194)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
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Results in Physics, 2018 The work presents microspectroscopic studies of the caries development and phase formation in dentin tissues using the methods of laser-induced fluorescence (LIF) and Raman spectroscopy. The results show that the data obtained near the boundary of intact
D.L. Goloshchapov +4 more
doaj +1 more source
Threonine 348 regulates the subcellular localization of PTEN
FEBS Open Bio, EarlyView.Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley +1 more source
Laser-induced fluorescence measurements on CdSe quantum dots [PDF]
Processing and Application of Ceramics, 2010 In this paper, we report on photoluminescence decay measurements on CdSe quantum dots (QDs) as a function of size in the diameter range of 2.1 to 3.5 nm. The nanoparticles were synthesized by the kinetic growth method from CdO and elemental Se precursors.
Zoltan Győri +6 more
doaj
MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation
FEBS Open Bio, EarlyView.MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima +8 more
wiley +1 more source
PhotonicsThis paper presents the results of an experimental study of the dynamic characteristics of the process of laser fragmentation/laser-induced fluorescence (LF/LIF) of trinitrotoluene traces on a paper surface under synchronized double-pulse laser ...
Sergei Bobrovnikov +2 more
doaj +1 more source
Journal of Spectroscopy, 2013 Distortion of laser-induced fluorescence profiles attributable to optical absorption and saturation broadening was corrected in combination with laser absorption spectroscopy in argon plasma flow.
S. Nomura +4 more
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Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells
FEBS Open Bio, EarlyView.Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley +1 more source
β‐Catenin/c‐Myc Axis Modulates Autophagy Response to Different Ammonia Concentrations
Advanced Biology, Volume 9, Issue 3, March 2025.Ammonia, detoxified by the liver into urea and glutamine, impacts autophagy differently at varying levels. Low ammonia activates autophagy via c‐Myc and β‐catenin, while high levels suppress it. Using Huh7 cells and Spf‐ash mice, c‐Myc's role in cytoprotective autophagy is revealed, offering insights into hyperammonemia and potential therapeutic ...
S. Sergio +11 more
wiley +1 more source
IEEE Photonics Journal, 2013 We simultaneously observe the probe transmission, four-wave mixing (FWM) and fluorescence signals under dressing effects in a four-level -type atomic system for the first time.
Zhiguo Wang +6 more
doaj +1 more source