Results 21 to 30 of about 74,397 (214)

The possibility of using laser and micro-jet technology in the welding of structural elements of vehicles [PDF]

Scientific Journal of Silesian University of Technology. Series Transport, 2015
A paper presents the possibility of laser welding using micro-jet cooling. The effect of micro-jet cooling on microstructure and mechanical properties of the weld metal deposit was carried out.
Wojciech MAJEWSKI
doaj  

Extracellular vesicle lipidomics liquid biopsy reveals systemic and tumor microenvironment metabolic reprogramming in ovarian cancer

Molecular Oncology, EarlyView.
Extracellular vesicle (EV) lipidomic profiling of plasma and ascites from patients with high‐grade serous ovarian cancer and of ovarian cancer cell lines reveals enrichment of triglycerides (TGs), diacylglycerols (DGs), phosphatidylcholine (PCs), sphingomyelins (SMs), reflecting tumor metabolic reprogramming.
Shikha Rani, Dominic Guanzon, Andrew Lai, Katherin Scholz‐Romero, Sarah Reed, Edita Ritmejeryte, Rohan Lourie, Naven Chetty, Kaltin Ferguson, Carlos Salomon   +9 more
wiley   +1 more source

Analysis of the Strength and toughness of welded joints in ultra high strength steel with a step-cooling laser welding

Hanjie xuebao
To address the strength-toughness mismatch in ultra-high strength steel welded joints, a step-cooling process inspired by the Quenching and Partitioning (Q&P) principle was proposed and its impact on the mechanical properties of welded joints was ...
Zhiyong ZHANG, Ruoyu LI, Xuanming GUO, Ruxing SHI, Xingsheng YU, Jijin XU, Zhimin LI   +6 more
doaj   +1 more source

Effect of Heat Sink and Cooling Mediums on Ferrite Austenite Ratio and Distortion in Laser Welding of Duplex Stainless Steel 2205

Chinese Journal of Mechanical Engineering, 2019
In order to control the ferrite and austenite percentage in duplex stainless steel welding, many researchers try to change the laser welding parameters and cooling medium, but ignore to study the influence of heat sink effect on weld strength.
P. Dinesh Babu, P. Gouthaman, P. Marimuthu   +2 more
doaj   +1 more source

Hybrid Laser Deposition of Fe-Based Metallic Powder under Cryogenic Conditions

Metals, 2020
The purpose of this study was to demonstrate the novel technique of laser deposition of Fe-based powder under cryogenic conditions provided by a liquid nitrogen bath.
Aleksander Lisiecki, Dawid Ślizak
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing Laser Sintering Parameters and Cooling Channel Designs for Enhanced Injection Molding of Medical Tube Connectors

Journal of Applied Science and Engineering
Respiratory equipment disconnections in hospitals pose severe risks, including hypoxia and mortality. Suboptimal tube connector quality, often worsened by traditional cooling mold designs, results in uneven thermal equilibrium, product deformation, and ...
Ding Yang Hsu
doaj   +1 more source

Three-dimensional model of small signal free-electron lasers

Physical Review Special Topics. Accelerators and Beams, 2011
Coherent electron cooling is an ultrahigh-bandwidth form of stochastic cooling which utilizes the charge perturbation from Debye screening as a seed for a free-electron laser.
Stephen Webb, Gang Wang, Vladimir Litvinenko   +2 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source