Results 71 to 80 of about 2,483,144 (287)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

A comparative evaluation of DIAGNOdent with visual and radiography for detection of occlusal caries: An in vitro study

Indian Journal of Dental Research, 2009
Background: The diagnosis of dental caries is fundamental to the practice of dentistry. Despite the fact that dental caries has declined considerably, it is still a problem of great importance.
Sridhar N, Tandon S, Rao Nirmala
doaj  

Laser-induced fluorescence of the CHF radical

Chemical Physics Letters, 1980
Abstract Laser-induced fluorescence of the A 1A - X 1A' transition in the gas-phase CHF radical has been observed, following the formation of the ground-state radical by IR multiple-photon dissociation. Radiative lifetimes of four vibronic states of CHF (A 1A) are reported.
Ashfold, Michael N R, CASTANO, F, HANCOCK, G, KETLEY, GW   +3 more
openaire   +1 more source

Laser-induced fluorescence determination of flame temperatures in comparison with CARS measurements [PDF]

, 1990
Lawitzki A, Plath I, Stricker W, Bittner J, Meier U, Kohse-Höinghaus K. Laser-induced fluorescence determination of flame temperatures in comparison with CARS measurements. Applied Physics, B.
Kohse-Höinghaus, K., Lawitzki, A., Lawitzki, Annette, Kohse-Höinghaus, Katharina, Meier, U., Meier, Ulrich, Stricker, W., Plath, I., Bittner, Jürgen, Bittner, J., K. Kohse-H�inghaus, A. Lawitzki, J. Bittner, I. Plath, W. Stricker, U. Meier   +15 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Low-loss Ti:sapphire waveguides fabricated by pulsed laser deposition

, 2012
We report the fabrication and characterisation of Ti:sapphire films epitaxially grown on c-cut sapphire substrates by pulsed laser deposition (PLD). Deposition conditions have been studied extensively and optimised in order to produce high-performance ...
Shepherd, D.P., Choudhary, Amol, Sposito, Alberto, Eason, R.W.   +3 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

The role of composition and preparation method on laser performance of holmium-doped silica fibers

Optical Materials: X
The composition of holmium-doped fibers has a fundamental influence on their fluorescence and laser properties. The same can apply for the preparation method, since it may affect the glass matrix in the close vicinity of active ions.
M. Kamrádek, R. Sajzew, J. Aubrecht, F. Lindner, P. Vařák, O. Podrazký, I. Bartoň, J. Bierlich, J. Dellith, I. Kašík, K. Wondraczek, P. Peterka, P. Honzátko   +12 more
doaj   +1 more source

Effect of Absorption and Scattering on Fluorescence of Buried Tumours

Journal of Spectroscopy, 2020
Fluorescence spectroscopy is widely used for biomedical optical diagnosis and surgical resection of tumours. This work investigates laser-induced fluorescence spectroscopy of fluorescence inclusions that are embedded in turbid media.
Kawthar M. K. Alghourani, Wesam Bachir, George Karraz   +2 more
doaj   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source