Results 61 to 70 of about 2,859,854 (215)

Analyzing and relieving the thermal issues caused by fabrication details of a deuterium cryogenic target

Matter and Radiation at Extremes, 2021
In inertial confinement fusion experiments, fuel quality is determined mainly by the thermal environment of the capsule in the layering procedure. Owing to the absence of a radial thermal gradient, formed deuterium–deuterium (DD) ice shells in the ...
Hong Yang, Shasha Gao, Baibin Jiang, Jun Xie, Juxi Liang, Xiaobo Qi, Kai Wang, Chaoyou Tao, Fei Dai, Wei Lin, Juan Zhang   +10 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Measurement of the sound velocity of shock compressed water

Scientific Reports, 2021
The sound velocities of water in the Hugoniot states are investigated by laser shock compression of precompressed water in a diamond anvil cell. The obtained sound velocities in the off-Hugoniot region of liquid water at precompressed conditions are used
Hua Shu, Jiangtao Li, Yucheng Tu, Junjian Ye, Junyue Wang, Qili Zhang, Huiru Tian, Guo Jia, Zhiyu He, Fan Zhang, Zhiyong Xie, Xiuguang Huang, Wenbin Pei, Sizu Fu   +13 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

2D longitudinal modeling of heat transfer and fluid flow during multilayered [PDF]

, 2012
version post-print de l'article : JLA Vol : 24 Iss:3. 2D longitudinal modeling of heat transfer and fluid flow during multilayered direct laser metal deposition processDerived from laser cladding, the Direct Laser Metal Deposition (DLMD) process is ...
CARRON, Denis, LE MASSON, Philippe, GHARBI, Myriam, PEYRE, Patrice, FABBRO, Rémy, CARIN, Muriel, MORVILLE, Simon   +6 more
core  

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Laser-induced breakdown of an optically trapped gold nanoparticle for single cell transfection [PDF]

, 2013
The cell selective introduction of therapeutic agents remains a challenging problem. Here we demonstrate spatially controlled cavitation instigated by laser-induced breakdown of an optically trapped single gold nanoparticle of diameter 100 nm. The energy
Gunn-Moore, Frank J, Arita, Yoshihiko, Antkowiak, Maciej, Ploschner, Martin, Dholakia, Kishan, Yoshihiko Arita, Maciej Antkowiak, Frank Gunn-Moore, Gunn-Moore, Frank, Martin Ploschner, Kishan Dholakia   +10 more
core   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

Understanding the Burden of Orofacial Involvement and Patient Treatment Preferences in Systemic Sclerosis: Results From a Large International Survey

Arthritis Care &Research, EarlyView.
Objective Orofacial manifestations are significantly impactful in patients with systemic sclerosis (SSc) yet remain understudied, with no dedicated clinical guidelines to inform their management. Methods An international online survey comprised38 questions addressing orofacial manifestations of SSc, including patients’ confidence in their treating ...
Eleni Deligianni, Lee Shapiro, Deana Gibbs, Zsuzsanna H. McMahan, Michael Hughes   +4 more
wiley   +1 more source

Interpretation in quantum physics as hidden curriculum [PDF]

, 2010
Prior research has demonstrated how the realist perspectives of classical physics students can translate into specific beliefs about quantum phenomena when taking an introductory modern physics course.
Baily, Charles, Finkelstein, Noah D., Mel Sabella, Charles Baily, Sanjay Rebello, Noah D. Finkelstein, Chandralekha Singh   +6 more
core   +1 more source