We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Luminance-Corrected 3D Point Clouds for Road and Street Environments
A novel approach to evaluating night-time road and street environment lighting conditions through 3D point clouds is presented. The combination of luminance imaging and 3D point cloud acquired with a terrestrial laser scanner was used for analyzing 3D ...
Matti T. Vaaja +6 more
doaj +1 more source
AUTOMATIC TEXTURE MAPPING OF ARCHITECTURAL AND ARCHAEOLOGICAL 3D MODELS [PDF]
Today, detailed, complete and exact 3D models with photo-realistic textures are increasingly demanded for numerous applications in architecture and archaeology.
T. P. Kersten, D. Stallmann
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
New Advances in Automated Urban Modelling from Airborne Laser Scanning Data [PDF]
Traditionally, urban models in many applications such as urban planning, disaster management, and computer games only require visual accuracy. However, more recently, updating urban infrastructure combined with the rise of mega-cities (i.e.
Hinks, Tommy +7 more
core +1 more source
Study of a High Spectral Resolution Hyperspectral LiDAR in Vegetation Red Edge Parameters Extraction
Non-contact and active vegetation or plant parameters extraction using hyperspectral information is a prospective research direction among the remote sensing community.
Changhui Jiang +9 more
doaj +1 more source
THE SELIMIYE MOSQUE OF EDIRNE, TURKEY – AN IMMERSIVE AND INTERACTIVE VIRTUAL REALITY EXPERIENCE USING HTC VIVE [PDF]
Recent advances in contemporary Virtual Reality (VR) technologies are going to have a significant impact on veryday life. Through VR it is possible to virtually explore a computer-generated environment as a different reality, and to immerse oneself into
T. P. Kersten +6 more
doaj +1 more source
Ancient lowland Maya complexity as revealed by airborne laser scanning of northern Guatemala
Classic Maya civilization in detail Lidar (a type of airborne laser scanning) provides a powerful technique for three-dimensional mapping of topographic features.
Marcello Canuto +17 more
semanticscholar +1 more source
Threonine 348 regulates the subcellular localization of PTEN
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley +1 more source
MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima +8 more
wiley +1 more source

