Results 61 to 70 of about 373,941 (268)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Interest point detection from multi‐beam light detection and ranging point cloud using unsupervised convolutional neural network

IET Image Processing, 2021
Interest point detection plays an important role in many computer vision applications. This work is motivated by the light detection and ranging odometry task in autonomous driving.
Deyu Yin, Qian Zhang, Jingbin Liu, Xinlian Liang, Yunsheng Wang, Shoubin Chen, Jyri Maanpää, Juha Hyyppä, Ruizhi Chen   +8 more
doaj   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

Multisource Single-Tree Inventory in the Prediction of Tree Quality Variables and Logging Recoveries

Remote Sensing, 2014
The stem diameter distribution, stem form and quality information must be measured as accurately as possible to optimize cutting. For a detailed measurement of the stands, we developed and demonstrated the use of a multisource single-tree inventory (MS ...
Mikko Vastaranta, Ninni Saarinen, Ville Kankare, Markus Holopainen, Harri Kaartinen, Juha Hyyppä, Hannu Hyyppä   +6 more
doaj   +1 more source

Scanning laser polarimetry in glaucoma

Indian Journal of Ophthalmology, 2014
Glaucoma is an acquired progressive optic neuropathy which is characterized by changes in the optic nerve head and retinal nerve fiber layer (RNFL). White-on-white perimetry is the gold standard for the diagnosis of glaucoma. However, it can detect defects in the visual field only after the loss of as many as 40% of the ganglion cells.
Tanuj Dada, Reetika Sharma, Dewang Angmo, Gautam Sinha, Shibal Bhartiya, Sanjay K Mishra, Anita Panda, Ramanjit Sihota   +7 more
openaire   +3 more sources

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

β‐Catenin/c‐Myc Axis Modulates Autophagy Response to Different Ammonia Concentrations

Advanced Biology, Volume 9, Issue 3, March 2025.
Ammonia, detoxified by the liver into urea and glutamine, impacts autophagy differently at varying levels. Low ammonia activates autophagy via c‐Myc and β‐catenin, while high levels suppress it. Using Huh7 cells and Spf‐ash mice, c‐Myc's role in cytoprotective autophagy is revealed, offering insights into hyperammonemia and potential therapeutic ...
S. Sergio, B. Spedicato, G. Corallo, A. Inguscio, M. Greco, D. Musarò, D. Vergara, A. F. Muro, G. De Sabbata, L. R. Soria, N. Brunetti Pierri, M. Maffia   +11 more
wiley   +1 more source

Quantitative Assessment of Scots Pine (Pinus Sylvestris L.) Whorl Structure in a Forest Environment Using Terrestrial Laser Scanning

IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing, 2018
State-of-the-art technology available at sawmills enables measurements of whorl numbers and the maximum branch diameter for individual logs, but such information is currently unavailable at the wood procurement planning phase.
Jiri Pyorala, Xinlian Liang, Mikko Vastaranta, Ninni Saarinen, Ville Kankare, Yunsheng Wang, Markus Holopainen, Juha Hyyppa   +7 more
doaj   +1 more source

Characterization of Defect Distribution in an Additively Manufactured AlSi10Mg as a Function of Processing Parameters and Correlations with Extreme Value Statistics

Advanced Engineering Materials, EarlyView.
Predicting extreme defects in additive manufacturing remains a key challenge limiting its structural reliability. This study proposes a statistical framework that integrates Extreme Value Theory with advanced process indicators to explore defect–process relationships and improve the estimation of critical defect sizes. The approach provides a basis for
Muhammad Muteeb Butt, Varma Kvvssn, Hossein Laieghi, Zia Uddin, Peyman Ansari, E. Kıvanç Sadak, Can Barış Toprak, Hüseyin Kızıl, Enrico Salvati   +8 more
wiley   +1 more source