Results 41 to 50 of about 180,806 (262)

An Inexpensive, 3D-Printable, Arduino- and Blu-Ray-Based Confocal Laser and Fluorescent Scanning Microscope

Metrology
There is a growing field that is devoted to developing inexpensive microscopes and measurement devices by leveraging low-cost commercial parts that can be controlled using smartphones or embedded devices, such as Arduino and Raspbery Pi. Examples include
Justin Loose, Samuel H. Hales, Jonah Kendell, Isaac Cutler, Ryan Ruth, Jacob Redd, Samuel Lino, Troy Munro   +7 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Microanatomy of the Rat Diaphragm: A Scanning Electron and Confocal Laser Scanning Microscopic Study.

Archives of Histology and Cytology, 1993
The present study demonstrated the three-dimensional microstructure of the rat diaphragm by scanning electron microscopy (SEM) of either intact or alkali-treated tissues, enzyme-histochemistry, and confocal laser scanning microscopy (LSM). The peritoneal and pleural surfaces of the diaphragm were covered with mesothelial cells studded with microvilli ...
Ohtani, Yuko, Ohtani, Osamu, Nakatani, Toshio   +2 more
openaire   +3 more sources

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

A Sensitive Thresholding Method for Confocal Laser Scanning Microscope Image Stacks of Microbial Biofilms

Scientific Reports, 2018
Biofilms are surface-attached microbial communities whose architecture can be captured with confocal microscopy. Manual or automatic thresholding of acquired images is often needed to help distinguish biofilm biomass from background noise.
Ting L. Luo, Marisa C. Eisenberg, Michael A. L. Hayashi, Carlos Gonzalez-Cabezas, Betsy Foxman, Carl F. Marrs, Alexander H. Rickard   +6 more
doaj   +1 more source

Antinuclear human autoantibodies as markers in Nicotiana tabacum pollen tubes

Acta Societatis Botanicorum Poloniae, 2014
In the present paper we report on the use of antinuclear human autoantibodies as specific markers in Nicotiana tabacum pollen tubes. The antibodies have been tested by fluorescence techniques using a confocal laser scanning microscope. All the antibodies
C. Poggialini, G. Morozzi, R. Marcolongo, R. Vignani, M. Cresti, A. Tiezzi   +5 more
doaj   +1 more source

β‐Catenin/c‐Myc Axis Modulates Autophagy Response to Different Ammonia Concentrations

Advanced Biology, Volume 9, Issue 3, March 2025.
Ammonia, detoxified by the liver into urea and glutamine, impacts autophagy differently at varying levels. Low ammonia activates autophagy via c‐Myc and β‐catenin, while high levels suppress it. Using Huh7 cells and Spf‐ash mice, c‐Myc's role in cytoprotective autophagy is revealed, offering insights into hyperammonemia and potential therapeutic ...
S. Sergio, B. Spedicato, G. Corallo, A. Inguscio, M. Greco, D. Musarò, D. Vergara, A. F. Muro, G. De Sabbata, L. R. Soria, N. Brunetti Pierri, M. Maffia   +11 more
wiley   +1 more source

Carboxylic‐Acid Functionalized Multiwalled Carbon Nanotube‐Alkane‐Based Resistive Temperature Sensor for Cold Chain Applications

Advanced Engineering Materials, EarlyView.
This study presents a reversible temperature sensor with high switching ratio, ∼103. The device is fabricated using PET‐ITO and carbon nanotube dispersions in alkane. Considering its application in cold chain logistics, a proof‐of‐concept with LED is showcased. Thus, a temperature drop below the threshold temperature (crystallization temperature of the
Sunil Kumar Behera, Philippe Marrec, A. Jorge Parola, Madalena Dionísio, Hugo Águas, Andreia F. M. Santos, Hamed Pourkheirollah, Donald Lupo, César A. T. Laia   +8 more
wiley   +1 more source

Design of a quantum diamond microscope with efficient scanning confocal readout

Academia Quantum
In this paper we introduce the light-sheet confocal quantum diamond microscope (LC-QDM) for widefield 3D quantum sensing with efficient confocal readout.
Daniel G. Ang, Jiashen Tang, Ronald L. Walsworth   +2 more
doaj   +1 more source