Results 151 to 160 of about 3,965 (211)

A 3D Human Bone and Bone Marrow‐on‐a‐Chip Model for In Vitro Bone Remodeling and Immune Cell Maintenance

Advanced Science, EarlyView.
This study presents a human Bone (and Bone Marrow)‐on‐a‐Chip model based on native human bone scaffolds and autologous cells. Dynamic perfusion and sequential cell seeding replicate the physiological bone remodeling process in vitro, enabling the long‐term culture of functional, mature bone marrow immune subpopulations.
Nina Stelzer, Melanie‐Jasmin Ort, Kristian Händler, Emely Bortel, Ioanna Maria Dimitriou, Martin Textor, Georg N. Duda, Janosch Schoon, Uwe Marx, Uwe Kornak, Annika Winter, Bernhard Hesse, Stefanie Donner, Sebastian Hardt, Oliver Klein, Simon Reinke, Malte Spielmann, Sven Geißler   +17 more
wiley   +1 more source

PRC2.1 Coordinates Peri‐Nucleolar H3K27me3‐Enriched Heterochromatin Organization and NPM1 Pentamerization to Maintain Nucleolar Integrity

Advanced Science, EarlyView.
PRC2.1(PCL2)‐coordinated H3K27me3‐enriched PNH establishes a spatial scaffold crucial for nucleolar integrity. As a crucial coordinator, PCL2 links PRC2.1 to chromatin organization and NPM1 assembly. This network‐based model reveals how chromatin modifications and nucleolar components cooperatively maintain nucleolar architecture, revealing novel ...
Lina Zhu, Wenqin Wang, Shuyun Chen, Gangyi Zhu, Zhen Wu, Yue Gu, Jingwen Xiong, Xu Zhang, Heng Liu, Kyoichi Isono, Youming Zhang, Jingjing Cao, Xiangzhi Li   +12 more
wiley   +1 more source

A Soft, Flexible Implant for Wireless Photothermal–Pyroelectric Neurostimulation

Advanced Science, EarlyView.
A replication strategy is used to enlarge the exposed surface area of barium titanate within flexible films with embedded carbon nanotubes, enabling efficient photothermal‐pyroelectric stimulation. Polar crystalline nanosheets show superior performance in guiding neural progenitor cells toward neuronal differentiation.
Jiang Wu, Minmin Mao, Beltzane Garcia Cirera, Hao Ye, Xiangzhong Chen, Josep Puigmartí‐Luis, Ni Qin, Salvador Pané   +7 more
wiley   +1 more source

Mitochondria and Neuromast Tagging With Fluorescent Gallium‐Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging

Advanced Science, EarlyView.
Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green, Michael W. Jones, Merissa Saleem, Haobo Ge, Melita Tvardauskaite, Julia Dudzic, Fernando Cortezon‐Tamarit, Rory L. Arrowsmith, Charareh Pourzand, Gabriele Kociock‐Kohn, Nicholas H. Rees, Jonathan R. Dilworth, Stephen Faulkner, Stanley W. Botchway, David Gurevich, Sofia I. Pascu   +15 more
wiley   +1 more source

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Modern Laser Scanning Confocal Microscopy

Current Protocols in Cytometry, 2018
AbstractSince its commercialization in the late 1980's, confocal laser scanning microscopy (CLSM) has since become one of the most prevalent fluorescence microscopy techniques for three‐dimensional structural studies of biological cells and tissues. The flexibility of the approach has enabled its application in a diverse array of studies, from the fast
Peter Bayguinov, James Fitzpatrick
exaly   +3 more sources

Laser scanning confocal fluorescence microscopy: an overview

International Immunopharmacology, 2003
Innovative and important aspects of laser scanning confocal fluorescence imaging (LSCFI) are presented here as a general overview. We have described and discussed the technology of the procedure in some detail. We also report some of our original work with transmembranous uptake of 5S gamma-globulin on living human leukocytes as an example of one ...
Gernot P Tilz, Zeno Földes-Papp, Ulrike Demel   +2 more
exaly   +3 more sources

Confocal Laser Scanning Microscopy

Advances in clinical pathology : the official journal of Adriatic Society of Pathology, 2001
Immunoelectron microscopy and electron microscopic in situ hybridization are undoubtedly the best methods for following the dynamic changes of subcellular organelles; however, these techniques require specific tissue preparation and equipment. More recent developments include a more refined and sophisticated technique, confocal laser scanning ...
Akira Matsuno, Johbu Itoh, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe   +4 more
openaire   +3 more sources

TelePresence Confocal Laser Scanning Microscopy

Microscopy and Microanalysis, 2001
Abstract The advent of the Internet has allowed the development of remote access capabilities to a growing variety and number of microscopy systems. To date, the confocal microscope has not been included among these systems. At the California State University (CSU) Confocal Microscopy Core Facility, we have established a remote access
Janey H., Youngblom, James J., Youngblom, Jerry, Wilkinson   +2 more
openaire   +2 more sources

Applications of confocal laser scanning microscopy

Trends in Cell Biology, 1991
J. P. Bacon is at the Neuroscience Interdisciplinary Research Centre, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK; C. Gonzalez is at the Department of Biochemistry and C. J. Hutchison is at the Department of Biological Sciences, University of Dundee, Dundee DD1 4HN, UK.
J P, Bacon, C, Gonzalez, C J, Hutchinson
openaire   +2 more sources

Advanced Microscopy: Laser Scanning Confocal Microscopy

2011
Fluorescence microscopy is an important and fundamental tool for biomedical research. Optical microscopy is almost non-invasive and allows highly spatially resolved images of organisms, cells, macromolecular complexes, and biomolecules to be obtained. Generally speaking, the architecture of the observed structures is not significantly modified and the ...
Orla, Hanrahan, James, Harris, Chris, Egan   +2 more
openaire   +2 more sources