Results 41 to 50 of about 2,911,025 (240)

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source

Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques

Journal of Imaging, 2021
Several imaging techniques are used in biological and biomedical studies. Micro-computed tomography (micro-CT) is a non-destructive imaging technique that allows the rapid digitisation of internal and external structures of a sample in three dimensions ...
Kleoniki Keklikoglou, Christos Arvanitidis, Georgios Chatzigeorgiou, Eva Chatzinikolaou, Efstratios Karagiannidis, Triantafyllia Koletsa, Antonios Magoulas, Konstantinos Makris, George Mavrothalassitis, Eleni-Dimitra Papanagnou, Andreas S. Papazoglou, Christina Pavloudi, Ioannis P. Trougakos, Katerina Vasileiadou, Angeliki Vogiatzi   +14 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Confocal laser scanning microscopy of nanoparticles applied to immunosorbent assays [PDF]

, 2011
The aim of this project was to demonstrate and develop a confocal readout method for fluorescent immunosorbent assays and investigate its potential advantages in comparison to traditional immunoassays.
Ghafari, H
core  

Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy

Bio-Protocol, 2018
Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display
Natalia Cattelan, Osvaldo Yantorno, Rajendar Deora   +2 more
doaj   +1 more source

Instantaneous Clearing of Biofilm (iCBiofilm): an optical approach to revisit bacterial and fungal biofilm imaging

Communications Biology, 2023
A new clearing and imaging technique termed iCBiofilm is developed for imaging whole bacterial and fungal biofilms using confocal laser scanning microscopy, which enables examining the dynamics and functions of biofilms.
Shinya Sugimoto, Yuki Kinjo
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells [PDF]

FEBS Open Bio
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Hink M.
europepmc   +2 more sources

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Confocal laser scanning microscopy in vivo for diagnosing melanocytic skin neoplasms

Vestnik Dermatologii i Venerologii, 2017
The authors discuss the use of confocal laser scanning microscopy in vivo (CLSM) for diagnosing melanocytic skin neoplasms and its value for early diagnostics of melanoma. CLSM is an innovation noninvasive visual examination method for real-time multiple
A. A. Kubanova, V. V. Chikin, YU. YU. Shtirshneider, O. R. Katunina   +3 more
doaj   +1 more source

In vitro studies of Rickettsia-host cell interactions: Confocal laser scanning microscopy of Rickettsia helvetica-infected eukaryotic cell lines. [PDF]

PLoS Neglected Tropical Diseases, 2018
Rickettsia (R.) helvetica is the most prevalent rickettsia found in Ixodes ricinus ticks in Germany. Several studies reported antibodies against R. helvetica up to 12.5% in humans investigated, however, fulminant clinical cases are rare indicating a ...
Stephanie Speck, Tanja Kern, Karin Aistleitner, Meik Dilcher, Gerhard Dobler, Sandra Essbauer   +5 more
doaj   +1 more source