Results 61 to 70 of about 2,146 (214)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Deglaciation of the Colorado Rocky Mountains following the Last Glacial Maximum

Cuadernos de Investigación Geográfica, 2017
The availability of almost 180 cosmogenic-radionuclide (CRN) surface-exposure ages from moraine boulders and glacially polished bedrock surfaces makes possible an assessment of the timing and character of the local Last Glacial Maximum (LLGM) and ...
E.M. Leonard, B.J.B. Laabs, A.D. Schweinsberg, C.M. Russell, J.P. Briner, N.E. Young   +5 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Greenland Ice Cores Reveal a South‐To‐North Difference in Holocene Thermal Maximum Timings

Geophysical Research Letters
Holocene temperature evolution remains poorly understood. Proxies in the early and mid‐Holocene suggest a Holocene Thermal Maximum (HTM) where temperatures exceed the pre‐industrial, whereas climate models generally simulate monotonic warming.
Kaden C. Martin, Christo Buizert, Ed Brook, Olivia L. Williams, Jon S. Edwards, Ben Riddell‐Young, T. J. Fudge, Farhana Mederbel, Ross Beaudette, Jeff Severinghaus, Ikumi Oyabu, Kenji Kawamura, Marie Kirk, Iben Koldtoft, J. P. Steffensen, Thomas Blunier   +15 more
doaj   +1 more source

Deglaciation of the Laurentide Ice Sheet from the Last Glacial Maximum

Cuadernos de Investigación Geográfica, 2017
The last deglaciation of the Laurentide Ice Sheet (LIS) was associated with major reorganisations in the ocean-climate system and its retreat also represents a valuable analogue for understanding the rates and mechanisms of ice sheet collapse. This paper
Ch.R. Stokes
doaj   +1 more source

Glacial geomorphology of the Strait of Magellan ice lobe, southernmost Patagonia, South America

Journal of Maps, 2020
We present a geomorphic map of the landforms created by the Patagonian Ice Sheet during the local Last Glacial Maximum and perhaps prior glaciations in southernmost Patagonia. Building on prior work, the new mapping focuses in unprecedented detail on the
Rodrigo L. Soteres, Carly Peltier, Michael R. Kaplan, Esteban A. Sagredo   +3 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Wetlands at the Last Glacial Maximum: Distribution and methane emissions [PDF]

Geophysical Research Letters, 2002
The global distribution of potential wetlands and their methane (CH4) emissions at the present‐day and the Last Glacial Maximum (LGM) are estimated using a GCM simulation of LGM climate, a vegetation model, and simple algorithms for determining wetland area based on topography and soil moisture, and CH4 emissions based on ecosystem carbon turnover in ...
openaire   +3 more sources

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

More efficient North Atlantic carbon pump during the Last Glacial Maximum

Nature Communications, 2019
Atmospheric CO2 is governed by CO2 gains (e.g., via Southern Ocean outgassing) and losses (e.g., via North Atlantic absorption). Using a novel method to estimate air–sea CO2 exchange signals, the authors show that North Atlantic CO2 absorption became ...
J. Yu, L. Menviel, Z. D. Jin, D. J. R. Thornalley, G. L. Foster, E. J. Rohling, I. N. McCave, J. F. McManus, Y. Dai, H. Ren, F. He, F. Zhang, P. J. Chen, A. P. Roberts   +13 more
doaj   +1 more source