Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Correction to: Is it a hemangioma? A combined case report of soft tissue sarcomas mimicking infantile hemangioma. [PDF]
J Surg Case Repeuropepmc +1 more source
From laboratory to prototype: the last-mile issue in whispering gallery mode resonator-based devices
Simone Berneschi +5 more
openalex +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Correction to 'Unleashing high trans-substrate cleavage kinetics of Cas12a for nucleic acid diagnostics'. [PDF]
Nucleic Acids Reseuropepmc +1 more source
Overview of molecular signatures of senescence and associated resources: pros and cons
FEBS Open Bio, EarlyView.Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
wiley +1 more source
Nearly a century of discoveries in bacterial genetics and their continuing impact on the field. [PDF]
G3 (Bethesda)Baltrus DA, Merrikh H, Uecker H, Wong A.
europepmc +2 more sources
Molecular determinants of signal transduction in tropomyosin receptor kinases
FEBS Open Bio, EarlyView.Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley +1 more source
Correction: Development of a core outcome set for paediatric upper limb spasticity; a structured review protocol for a systematic review of the literature and identification of a core outcome set using a Delphi survey. [PDF]
TrialsLiddle A +5 more
europepmc +1 more source