Results 91 to 100 of about 1,748,641 (295)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Late-glacial and Holocene European pollen data

open access: yesJournal of Maps, 2017
The European Pollen Database (EPD) is a community effort to archive and make available pollen sequences from across the European continent. Pollen sequences provide records that may be used to infer past vegetation and vegetation change.
Simon Brewer   +12 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Using sea-level and land motion data to develop an improved glacial isostatic adjustment model for the British Isles [PDF]

open access: yes, 2011
The Glacial Isostatic Adjustment (GIA) of the British Isles is of interest due to the constraints that can be provided on key model parameters such as regional ice sheet history, viscoelastic earth structure and the global meltwater (eustatic) signal ...
Bradley, Sarah Louise   +1 more
core  

Geomorphology of the Kaikoura area [PDF]

open access: yes, 1968
The major physiographic units in the Kaikoura area are the Peninsula Block, Beach Ridges and Raised Beaches, Hard Rock Areas and the Alluvial Fans. Erosion of the Seaward Kaikoura Mountains and the transfer of the debris to the sea by fan streams have ...
Chandra, Satish
core   +1 more source

Last Glacial Maximum cooling induced positive moisture balance and maintained stable human populations in Australia

open access: yesCommunications Earth & Environment
Long-standing interpretations of the Last Glacial Maximum (21,000 ± 2000 years ago) in Australia suggest that the period was extremely cold and arid, during which the Indo-Australian summer monsoon system collapsed, and human populations declined and ...
Haidee Cadd   +7 more
doaj   +1 more source

Palaeoenvironmental record of the last 70 000 yr in San Felipe Basin, Sonora desert, Mexico: preliminary results [PDF]

open access: yesGeofísica Internacional, 1999
Preliminary results of a palaeoenvironmental research which includes particle size analyses, diatom and rock-magnetic analyses for a 9.5 m long core from Laguna Seca de San Felipe (LSSF), Baja California, are presented.
De la O Villanueva, Margarita   +3 more
doaj  

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

LATE GLACIAL AND HOLOCENE ENVIRONMENTAL CHANGE INFERRED FROM THE PÁRAMO OF CAJANUMA IN THE PODOCARPUS NATIONAL PARK, SOUTHERN ECUADOR

open access: yesCaldasia, 2014
To reconstruct the environmental history including vegetation, fire and climate dynamics, from the Cajanuma valley area ( 3285 m elevation) in the Podocarpus National Park, southern Ecuador , we address the following major research question: (1) How did ...
ANDREA VILLOTA, HERMANN BEHLING
doaj   +1 more source

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