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During the current opioid crisis, the majority of overdose deaths are attributed to ultra‐potent synthetic opioids, such as fentanyl. While fentanyl exerts its action via opioid receptors, we propose that potassium channels in the brain blood vessels may also sense fentanyl.
Shiwani Thapa +4 more
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Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
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Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi +6 more
wiley +1 more source
Student engagement extends beyond simple attendance. Across 891 bioscience students from three universities, attendance behaviour was associated mainly with structural influences, whereas classroom spatial behaviour reflected affective and sensory factors.
Nigel Page +4 more
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BIOLOGICAL CHLORINATION. VIII. LATE INTERMEDIATES IN THE BIOSYNTHESIS OF CALDARIOMYCIN.
J R, BECKWITH, L P, HAGER
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Late intermediates in the biosynthesis of narciclasine
Journal of the Chemical Society D: Chemical Communications, 1971Tracer experiments show the intermediacy of racemic noroxomaritidine (5), normaritidine (7), and crinine (4) in the biological conversion of O-methylnorbelladine (1) into narciclasine (11).
C. Fuganti, M. Mazza
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Difference Spectra Of Late Intermediates Of The Bacteriorhodopsin Photocycle
SPIE Proceedings, 1989The flash-induced difference absorbance spectra of bacteriorhodopsin (BR) were measured at various times after an actinic flash using a diode array spectrophotometer (300-700 nm). Difference spectra for three late bacteriorhodopsin photocycle intermediates Mfast (Mf), mslow (Ms) and R are reported.
R. Govindjee, Zs. Dancshazy, T. G. Ebrey
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Studies on the late steps of (+) pisatin biosynthesis: Evidence for (−) enantiomeric intermediates
Phytochemistry, 2006Pisatin, a 6a-hydroxyl-pterocarpan phytoalexin from pea (Pisum sativum L.), is relatively unique among naturally occurring pterocarpans by virtue of the (+) stereochemistry of its 6a-11a C-C bond. However, pisatin synthesizing pea tissue has an isoflavone reductase, first identified in alfalfa, which acts on the (-) antipode.
Gregory L, DiCenzo, Hans D, VanEtten
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Intermediate-band photometry of late-type stars.
Publications of the Astronomical Society of the Pacific, 1976In four-color, intermediate-band photometry of late-type stars, difficulty has been experienced in reproducing the Str5mgren system as defined by standard stars published by Crawford and Barnes. The difficulty is believed to be connected with the fact that an international, bulk order for filters has produced a considerably narrower V filter than that ...
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