Results 191 to 200 of about 178,173 (277)

The “Skipping Rope” Technique: A Novel Strategy for Distal Burr Entrapment During Rotational Atherectomy

open access: yesCatheterization and Cardiovascular Interventions, EarlyView.
ABSTRACT We describe the “skipping rope technique” for managing burr entrapment during rotational atherectomy. Sequential counterclockwise rotational force is applied to the drive shaft, generating alternative torque dynamics. This maneuver provides a safe method to disengage a stuck rotaburr, offering operators an additional strategy for this ...
Thomas J. Ford   +3 more
wiley   +1 more source

3D‐Printed Coil Immobilized Enzyme Reactors for Laccase Biocatalysis in Single‐ and Two‐Phase Flow

open access: yesChemie Ingenieur Technik, EarlyView.
Development of 3D‐printed immobilized enzyme reactors functionalized with laccase for continuous‐flow biocatalysis. Surface coupling and reactor design enhanced stability and mass transfer, enabling efficient and reusable biocatalytic processes.
Alina J. Tauriainen   +4 more
wiley   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

open access: yesCytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas   +5 more
wiley   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

open access: yesCytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen   +2 more
wiley   +1 more source

Advances, Challenges, and Perspectives in Glomalin-Related Soil Protein Research. [PDF]

open access: yesMicroorganisms
Ling Q   +14 more
europepmc   +1 more source

A Streamlined High Performance Liquid Chromatography with Tandem Mass Spectrometry Based Workflow for Rapid Screening of Cellular Accumulation of Small Molecules

open access: yesChemMedChem, EarlyView.
This study uses six small‐molecule inhibitors to evaluate optimal cellular accumulation assay conditions in a 96‐well format with high performance liquid chromatography with tandem mass spectrometry detection. The obtained medium to high‐throughput workflow is suitable for reliably identifying cellular accumulation, valuable for preclinical selection ...
Alina Metzen, Katharina Rox
wiley   +1 more source

Industrial Sustainability Policies: Systematic Literature Review and Research Directions

open access: yesCorporate Social Responsibility and Environmental Management, EarlyView.
ABSTRACT Public policies for industrial sustainability have proliferated in recent years, leading to a significant increase in research on the topic. Nevertheless, these studies remain fragmented, and a holistic, up‐to‐date map of research integrating current knowledge from the definition of the agenda setting and policy design to policy evaluation ...
Nunzia Zecchillo   +2 more
wiley   +1 more source

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