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Lensless Digital Holographic Microscopy for Microbe Detection

Imaging and Applied Optics 2016, 2016
Microbial life on ocean worlds can be searched for with microscopes capable of tracking 3-dimensional particle motion. Here we discuss several lensless digital holographic microscope designs that can enable micron-scale resolution in very compact packages.
Eugene Serabyn   +5 more
openaire   +1 more source

Subpixel-Resolution in Digital Lensless Microscopy

Optica Imaging Congress 2025 (3D, DH, COSI, IS, pcAOP,RadIT)
We present a simulation study demonstrating subpixel-resolution phase retrieval in lensless microscopy by modeling pixel binning and object support constraints, enabling accurate recovery from highly undersampled diffraction intensity measurements.
Ritish Kamboj, Satish Kumar Dubey
openaire   +1 more source

Color lensless digital holographic microscopy with micrometer resolution

Optics Letters, 2012
Color digital lensless holographic microscopy with micrometer resolution is presented. Multiwavelength illumination of a biological sample and a posteriori color composition of the amplitude images individually reconstructed are used to obtain full-color representation of the microscopic specimen.
openaire   +2 more sources

Lensless phase contrast microscopy based on multiwavelength Fresnel diffraction

Optics Letters, 2014
We demonstrate a compact, wide-field, quantitative phase contrast microscope that does not require lenses for image formation. High-resolution images are retrieved from Fresnel diffraction patterns recorded at multiple wavelengths, combined with a robust iterative phase retrieval algorithm.
Daniel W. E. Noom   +2 more
openaire   +3 more sources

Evaluating autofocusing metrics in digital lensless holographic microscopy

Optics and Photonics for Advanced Dimensional Metrology III
This study proposes a novel approach in response to the persistent challenge of achieving precise autofocus in Digital Lensless Holographic Microscopy (DLHM). It involves employing an enhanced Bluestein algorithm to simulate DLHM holograms under a variety of conditions, spanning amplitude-only, phase-only, and amplitude-phase objects.
Lopera, Maria J.   +4 more
openaire   +2 more sources

Microscopy based on digital lensless Fourier transforms holography

SPIE Proceedings, 2010
With the recent technological advances, there is an increasing need for testing the microstructure of objects. We show here Digital Holographic Microscopy (DHM) that implements digitally the principle of holography. It is particularly well suited for recording and reconstructing three-dimensional objects. In this paper, we present an off-axis digital
Zhaoji Chen   +3 more
openaire   +1 more source

An Introduction to Lensless Digital Holographic Microscopy

2018
Digital Holographic Microscopy (DHM) is a technique that uses optical interference patterns to record a three-dimensional optical field for imaging, sensing, and microscopy applications. “Lensless” in-line DHM is the simplest arrangement, requiring no lenses, no mirrors, and typically only a light source, sample, and a digital imager chip such as a CCD
openaire   +1 more source

Detection of trichomonal vaginalis through lensless optofluidic microscopy

Advances in Microscopic Imaging II, 2019
Trichomoniasis is one of the most common non-viral sexually transmitted infection caused by a parasite called Trichomonas vaginalis. Currently, there is no timely, cost-efficient diagnostic test exist for Trichomoniasis. We present a lensless optofluidic imaging technique for label-free point-of-care detection of motile parasites in bodily fluids. With
Jessica Kun   +3 more
openaire   +1 more source

Improving color lensless microscopy reconstructions by self-calibration

Unconventional Optical Imaging, 2018
Lensless color microscopy is a recent 3D quantitative imaging method allowing to retrieve physical parameters characterizing microscopic objects spread in a volume. The main advantages of this technique are related to its simplicity, compactness, low sensitivity of the setup to vibrations and the possibility to accurately characterize objects. The cost-
Fabien Momey   +4 more
openaire   +1 more source

Hologram digital correction for Digital lensless Holographic Microscopy

Frontiers in Optics / Laser Science, 2020
A preprocessing method for Digital lensless Holographic Microscopy based on the resizing and sharpening of the holograms prior reconstruction is presented. This proposal has been successfully validated using experimental holograms.
openaire   +1 more source

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