Results 71 to 80 of about 5,400,299 (303)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

The roles of nucleation and growth kinetics in determining Li metal morphology for Li metal batteries: columnar versus spherical growth

, 2022
Li metal thin films have been considered one of the ideal anode materials for high energy density rechargeable batteries. Although recent efforts have shown promise in improving the electrochemical performance of Li metal, current Li metal technology ...
Oh, Jeongeun, Kwon, Bomee, Jo, Seunghyeon, Lee, Kyu Tae, Park, Kyobin, Lee, Jeonghyeop   +5 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Plasma grown fluoride-rich artificial SEI for stabilizing Li metal anodes

, 2023
The growth and detachment of lithium (Li) dendrites are regarded as the primary issues hindering the practical application of Li metal anodes, and constructing a stable solid electrolyte interface (SEI) is considered to be the most straightforward ...
Feng, TT, Zhou, HP, Fang, ZX, Zhang, S, Xu, ZQ, Wang, HM, Wu, MQ, Zhang, H   +7 more
core   +1 more source

A Li-free inverted-stack all-solid-state thin film battery using crystalline cathode material

Electrochemistry Communications, 2019
Lithium metal is a potential anode material to develop all-solid-state battery (SSB) with high energy density. However, Li metal easily reacts with atmospheric component (H2O, CO2, O2, N2) and then Li metal must be treated sensitively for industrial ...
Takayuki Yamamoto, Hiroki Iwasaki, Yasuhiro Suzuki, Miyuki Sakakura, Yasuhisa Fujii, Munekazu Motoyama, Yasutoshi Iriyama   +6 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Bi-containing Electrolyte Enables Robust and Li Ion Conductive Solid Electrolyte Interphase for Advanced Lithium Metal Anodes

Frontiers in Chemistry, 2020
The notorious lithium dendrite growth, causing the safety concern, hinders the practical application of high-capacity Li metal anodes for rechargeable batteries.
Yongliang Cui, Sufu Liu, Bo Liu, Donghuang Wang, Yu Zhong, Xuqing Zhang, Xiuli Wang, Xinhui Xia, Changdong Gu, Jiangping Tu   +9 more
doaj   +1 more source