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Laboratory Protocols for Candidatus Liberibacter asiaticus Transmission Biology and Management Research. [PDF]

Curr Protoc
Abstract Candidatus Liberibacter asiaticus (CLas), the causal agent of Huanglongbing, is transmitted by the Asian citrus psyllid (Diaphorina citri) in a persistent, propagative manner. Reproducible transmission studies require not only controlled inoculation assays but also standardized propagation of citrus host plants, maintenance of D.
Galdeano DM, Ingram W, Erickson A, Carlson CR, Falk BW, Kuo YW.   +5 more
europepmc   +2 more sources

Identification of the genes required for the culture of Liberibacter crescens, the closest cultured relative of the uncultured Liberibacter plant pathogens [PDF]

Frontiers in Microbiology, 2016
Here Tn5 random transposon mutagenesis was used to identify the essential elements for culturing Liberibacter crescens BT-1 that can serve as antimicrobial targets for the closely related pathogens of citrus, Candidatus Liberibacter asiaticus (Las) and ...
Kin-Kwan eLai, Austin G Davis-Richardson, Raquel eDias, Eric W. Triplett   +3 more
doaj   +4 more sources

Screening of diverse Psylloidea species in Taiwan reveals the presence of both known and potentially novel “Candidatus Liberibacter” species in multiple psyllid lineages [PDF]

Microbiology Spectrum
Psyllids (Psylloidea) are host-specific, phloem-feeding insects that are associated with the transmission of destructive plant diseases caused by the bacterial genus “Candidatus Liberibacter”.
Reun-Ping Goh, Yi-Chang Liao, Man-Miao Yang, Chia-Ching Chu   +3 more
doaj   +2 more sources

High-efficiency genome-editing, transgene evaluation, and antimicrobial efficacy testing using Citrus medica L. hairy roots. [PDF]

Plant J
Significance Statement Huanglongbing (HLB) or citrus greening disease threatens global citrus production, yet progress toward effective resistance strategies has been limited by the causal pathogen's unculturable nature and the difficulty of transforming citrus plants.
Kulshreshtha A, Ramasamy M, Irigoyen S, Padilla CS, Tu CK, Laughlin K, Borneman J, Mandadi KK.   +7 more
europepmc   +2 more sources

Genomic footprint of a shared Type 5 prophage in “Candidatus Liberibacter asiaticus” and “Candidatus Liberibacter africanus,” two destructive bacterial pathogens of citrus Huanglongbing [PDF]

Applied and Environmental Microbiology
Huanglongbing (HLB) is a bacterial disease that affects citrus trees and is considered the most severe citrus disease in the world. The three HLB-associated “Ca.
Frédéric Labbé, Claudine Boyer, Fernando Clavijo-Coppens, Blandine Benoist, Patrick Turpin, Santatra Ravelomanantsoa, Olivier Pruvost   +6 more
doaj   +2 more sources

Host-free biofilm culture of “Candidatus Liberibacter asiaticus,” the bacterium associated with Huanglongbing

Biofilm, 2019
Inability to culture the phloem-restricted alpha-proteobacterium “Candidatus Liberibacter asiaticus” (“Ca. L. asiaticus”) or the closely related species (“Candidatus Liberibacter americanus” and “Candidatus Liberibacter africanus”) that are associated ...
Phuc T Ha, Nabil Killiny, Judith K Brown
exaly   +3 more sources

Genetic Signatures of Contrasted Outbreak Histories of “Candidatus Liberibacter asiaticus”, the Bacterium That Causes Citrus Huanglongbing, in Three Outermost Regions of the European Union [PDF]

Evolutionary Applications
In an era of trade globalization and climate change, crop pathogens and pests are a genuine threat to food security. The detailed characterization of emerging pathogen populations is a prerequisite for managing invasive species pathways and designing ...
Olivier Pruvost, Karine Boyer, Frédéric Labbé, Marine Weishaar, Anaïs Vynisale, Claire Melot, Cécile Hoareau, Gilles Cellier, Virginie Ravigné   +8 more
doaj   +2 more sources

‘Candidatus liberibacter solanacearum’ protein CKC_05770 interacts in vivo with tomato APX6 and APX7 [PDF]

Scientific Reports
Pathogens have evolved mechanisms to manipulate their hosts to facilitate effective colonization and infection. One such mechanism involves the secretion of effectors that interfere with the host immune response.
Julien Gad Levy, Adwaita Prasad Parida, Junepyo Oh, Azucena Mendoza-Herrera, Brian D. Shaw, Cecilia Tamborindeguy   +5 more
doaj   +2 more sources

Huanglongbing as a Persistent Threat to Citriculture in Latin America [PDF]

Biology
Citrus commercial species are the most important fruit crops in the world; however, their cultivation is seriously threatened by the fast dispersion of emerging diseases, including Huanglongbing (HLB) citrus greening.
Jael Arely Cervantes-Santos, Hernán Villar-Luna, Ana Marlenne Bojórquez-Orozco, José Ernesto Díaz-Navarro, Ángela Paulina Arce-Leal, María Elena Santos-Cervantes, Manuel Gonzalo Claros, Jesús Méndez-Lozano, Edgar Antonio Rodríguez-Negrete, Norma Elena Leyva-López   +9 more
doaj   +2 more sources

Gamma-Aminobutyric Acid Accumulation Contributes to Citrus sinensis Response against ‘Candidatus Liberibacter Asiaticus’ via Modulation of Multiple Metabolic Pathways and Redox Status

Plants, 2023
Huanglongbing (HLB; also known as citrus greening) is the most destructive bacterial disease of citrus worldwide with no known sustainable cure yet. Herein, we used non-targeted metabolomics and transcriptomics to prove that γ-aminobutyric acid (GABA ...
Yasser Nehela, Nabil Killiny
doaj   +1 more source