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Bacterial Vaginosis (BV) involves the presence of a multi-species biofilm adhered to vaginal epithelial cells, but its in-depth study has been limited due to the complexity of the bacterial community, which makes the design of in vitro models challenging.
Joana Castro +5 more
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Optimizing a reliable ex vivo human blood model to analyze expression of Staphylococcus epidermidis genes [PDF]
Human blood is often used as an ex vivo model to mimic the environment encountered by pathogens inside the host. A significant variety of experimental conditions has been reported. However, optimization strategies are often not described.
Susana Brás +2 more
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Contiene: Título: Manifiestos feministas. Antología de textos del movimiento feminista español (1965-1985). Autora: Ménica Moreno Seco (ed.). Edita: Colección Lilith. Centro de Estudios sobre la Mujer de la Universidad de Alicante.
Andrés Fisher +2 more
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La poesía mapuche contemporánea escenifica una multiplicidad de procesos sufridos por este pueblo: desde las acometidas colonizadoras, hasta el desplazamiento forzoso y la reterritorialización en las periferias urbanas.
María Fernanda Libro
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Diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluorescence in situ hybridization method [PDF]
Bacterial vaginosis (BV) is one of most common vaginal infections. However, its diagnosis by classical methods reveals low specificity. Our goal was to evaluate the accuracy diagnosis of 150 vaginal samples with research gold standard methods and our ...
António Machado +4 more
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Candida albicans Adaptation on Simulated Human Body Fluids under Different pH
Candida albicans remains the most problematic of all Candida species, causing severe infections. Adaptation to different human body niches, such oral and urinary tracts, has been shown to be essential for survival and critical for virulence of C ...
Ana Barbosa +4 more
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Staphylococcus epidermidis is a major nosocomial pathogen with a remarkable ability to adhere to the surfaces of indwelling medical devices and form biofilms. Unlike other nosocomial pathogens, the interaction of S.
Fernando Oliveira +4 more
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Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates [PDF]
Staphylococcus epidermidis is one of the major opportunistic bacterial pathogens in healthcare facilities, mainly due to its strong ability to form biofilms in the surface of indwelling medical devices.
Vânia Gaio, Nuno Cerca
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Assessing and reducing sources of gene expression variability in Staphylococcus epidermidis biofilms
Gene expression quantification can be a useful tool in studying the properties of bacterial biofilms. Unfortunately, techniques such as RNA extraction, cDNA synthesis, and quantitative PCR (qPCR) can introduce variability into mRNA transcript ...
Cármen Sousa +2 more
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Pitfalls Associated with Discriminating Mixed-Species Biofilms by Flow Cytometry
Since biofilms are ubiquitous in different settings and act as sources of disease for humans, reliable methods to characterize and quantify these microbial communities are required.
Tânia Grainha +3 more
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