Results 81 to 90 of about 5,255,084 (312)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Methods of Molecular Analysis in the Life Sciences

, 2012
Delivering fundamental insights into the most popular methods of molecular analysis, this text is an invaluable resource for students and researchers.
Jones, M, Hofmann, A, Grkovic, T, Simon, A   +3 more
core  

UV Hyperspectral Imaging and Chemometrics for Honeydew Detection: Enhancing Cotton Fiber Quality

Chemosensors
Cotton, the most widely produced natural fiber, is integral to the textile industry and sustains the livelihoods of millions worldwide. However, its quality is frequently compromised by contamination, particularly from honeydew, a substance secreted by ...
Mohammad Al Ktash, Mona Knoblich, Frank Wackenhut, Marc Brecht   +3 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Evaluation of protein quantity and protein nutritional quality of protein bars with different protein sources

Scientific Reports
A comprehensive overview of commercially available protein bars, focusing on their protein content, protein source(s) and nutritional composition. Four protein bars were selected based on the quality of their protein sources; (i) plant only (pea and rice)
Judit Tormási, Eszter Benes, Éva Lengyel Kónya, Mária Berki, László Abrankó   +4 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

What Matters for Well-Being: Individual Perceptions of Quality of Life Before and After Important Life Events

, 2012
In recent decades, what matters for individual quality of life (QoL) has increasingly been the focus of empirical social science research. However, individuals are rarely asked directly what is important for their quality of life as part of large-scale ...

core  

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Hardtank® technology in extraction of caffeine and chlorogenic acid from coffee: analysis of chemical composition and antioxidant properties of coffee beverages

Frontiers in Chemistry
IntroductionHardtank® is a pressure-modulated, solvent-free cold extraction that shortens brewing while maximizing bioactive recovery from roasted coffee.MethodsWe produced a Nitro Cold Brew beverage from roasted Yellow Bourbon beans using Hardtank® (550
Radosław Kowalski, Kamil Belniak, Grażyna Kowalska, Marzena Włodarczyk-Stasiak, Artur Mazurek, Tomasz Baj   +5 more
doaj   +1 more source