Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Health worker perspectives on Greenlandic healthcare - resources, sustainability and work-life-balance. [PDF]
Aagaard T, Seibæk L.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Dispositional mindfulness is associated with lower smartphone addiction through digital life balance among Chinese university students. [PDF]
Aldbyani A +3 more
europepmc +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Correction: Dispositional mindfulness is associated with lower smartphone addiction through digital life balance among Chinese university students. [PDF]
Aldbyani A +3 more
europepmc +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Exploring nurse perspectives on AI-based shift scheduling for fairness, transparency, and work-life balance. [PDF]
Gerlach M +4 more
europepmc +1 more source
The impact of AI literacy on work-life balance and job satisfaction among university faculty: a self-determination theory perspective. [PDF]
Huang L, Zhao Y.
europepmc +1 more source
Work-life balance and effort-reward imbalance, and their interaction, associated with presenteeism among Korean wage workers: Based on 6th Korean working conditions survey. [PDF]
Kim SH +6 more
europepmc +1 more source

