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Native Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations

Chemical Reviews, 2019
The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester with an N-terminal cysteinyl peptide to produce a native peptide bond between the two fragments. This reaction has considerably extended the size of polypeptides
Vangelis Agouridas   +2 more
exaly   +2 more sources

Native chemical ligation in protein synthesis and semi-synthesis

Chemical Society Reviews, 2018
Native chemical ligation (NCL) provides a highly efficient and robust means to chemoselectively link unprotected peptide and protein segments to generate proteins. The ability to incorporate non-proteinogenic amino acids (e.g.d-amino acids or fluorescent
Anne C Conibear   +2 more
exaly   +2 more sources

Ligation Technologies for the Synthesis of Cyclic Peptides

Chemical Reviews, 2019
Cyclic peptides have been attracting a lot of attention in recent decades, especially in the area of drug discovery, as more and more naturally occurring cyclic peptides with diverse biological activities have been discovered.
Xuechen Li
exaly   +2 more sources

Serine/Threonine Ligation and Cysteine/Penicillamine Ligation

2022
Serine/threonine ligation (STL) and cysteine/penicillamine ligation (CPL) are highly chemo- and regioselective reactions between unprotected peptides with C-terminus salicylaldehyde esters and unprotected peptides with N-terminus serine/threonine or cysteine/penicillamine, which serve as powerful tools for cyclic peptide natural product and chemical ...
Jiamei, Liu, Xuechen, Li
openaire   +2 more sources

Subtiligase-Catalyzed Peptide Ligation.

Chemical Reviews, 2020
Subtiligase-catalyzed peptide ligation is a powerful approach for site-specific protein bioconjugation, synthesis and semisynthesis of proteins and peptides, and chemoproteomic analysis of cellular N termini.
A. Weeks, J. Wells
semanticscholar   +1 more source

Chemoselective Ligation in Glycochemistry

ChemInform, 2004
AbstractFor Abstract see ChemInform Abstract in Full Text.
PERI, FRANCESCO, NICOTRA, FRANCESCO
openaire   +4 more sources

An Elongation- and Ligation-Based qPCR Amplification Method for the Radiolabeling-Free Detection of Locus-Specific N6 -Methyladenosine Modification.

Angewandte Chemie, 2018
The epitranscriptomic mark N6 -methyladenosine (m6 A) is the most abundant RNA modification in eukaryotic mRNA, but various limitations in currently available m6 A detection methods have precluded routine identification of m6 A marks at the single-site ...
Yu Xiao   +5 more
semanticscholar   +1 more source

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