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Correlated Light Microscopy and Electron Microscopy
2012Understanding where, when, and how biomolecules (inter)act is crucial to uncover fundamental mechanisms in cell biology. Recent developments in fluorescence light microscopy (FLM) allow protein imaging in living cells and at the near molecular level. However, fluorescence microscopy only reveals selected biomolecules or organelles but not the (ultra ...
Sjollema, Klaas A. +4 more
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2006
Light microscopy is fundamental to the biomedical sciences. Although light microscopy is more than four centuries old, the design of the light microscope continues to evolve, meeting the demands of biomedical research. This chapter briefly introduces the basic theory of light microscopy. Issues to consider when selecting a light microscope for specific
Dawe, Gavin S. +4 more
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Light microscopy is fundamental to the biomedical sciences. Although light microscopy is more than four centuries old, the design of the light microscope continues to evolve, meeting the demands of biomedical research. This chapter briefly introduces the basic theory of light microscopy. Issues to consider when selecting a light microscope for specific
Dawe, Gavin S. +4 more
openaire +3 more sources
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 2004
Abstract This article provides information on the basic components of a light microscope, including the illumination system, collector lens, and optical and mechanical components. It describes optical performance in terms of image aberrations, resolution, and depth of field.
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Abstract This article provides information on the basic components of a light microscope, including the illumination system, collector lens, and optical and mechanical components. It describes optical performance in terms of image aberrations, resolution, and depth of field.
openaire +3 more sources
Light Microscopy Digital Imaging
Current Protocols in Cytometry, 2011AbstractThis unit presents an overview of digital imaging hardware used in light microscopy. CMOS, CCD, and EMCCDs are the primary sensors used. The strengths and weaknesses of each define the primary applications for these sensors. Sensor architecture and formats are also reviewed.
James, Joubert, Deepak, Sharma
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1984
Abstract This chapter discusses the tools and techniques of light microscopy and how they are used in the study of materials. It reviews the basic physics of light, the inner workings of light microscopes, and the relationship between resolution and depth of field.
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Abstract This chapter discusses the tools and techniques of light microscopy and how they are used in the study of materials. It reviews the basic physics of light, the inner workings of light microscopes, and the relationship between resolution and depth of field.
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Correlative light–electron microscopy
2011Recent advances in combining light and electron microscopy imaging techniques provide the means to correlate dynamic biological processes with the underlying structural correlates in situ. In this chapter, we provide snapshots of current advances targeting quantitative correlation of the dynamic state of a biological pathway with high-resolution ...
Dorit, Hanein, Niels, Volkmann
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Light Microscopy Digital Imaging
Current Protocols in Cytometry, 2008AbstractThis unit presents an overview of digital imaging hardware used in light microscopy. CMOS, CCD, and EMCCDs are the primary sensors used. The strengths and weaknesses of each define the primary applications for these sensors. Sensor architecture and formats are also reviewed.
openaire +2 more sources