Results 71 to 80 of about 1,606,955 (297)

Recruitment of the cellular lipid transport protein CERT to C. psittaci inclusions regulates the timing of bacterial egress

Scientific Reports
Egress of intracellular pathogens is highly regulated and carefully timed. For the zoonotic bacterium C. psittaci, the predominant egress pathway is Chlamydia-containing sphere (CCS) formation, a calcium-dependent sequential mechanism including protease ...
Jana Scholz, Gudrun Holland, Michael Laue, Sebastian Banhart, Dagmar Heuer   +4 more
doaj   +1 more source

Smad4 controls signaling robustness and morphogenesis by differentially contributing to the Nodal and BMP pathways

Nature Communications, 2021
The role of the transcriptional effector SMAD4 in vertebrate embryo development remains unresolved. Here the authors show that in the absence of Smad4, dorsal/ventral embryo patterning is disrupted due to the loss of BMP signaling, while Nodal signaling ...
Luca Guglielmi, Claire Heliot, Sunil Kumar, Yuriy Alexandrov, Ilaria Gori, Foteini Papaleonidopoulou, Christopher Barrington, Philip East, Andrew D. Economou, Paul M. W. French, James McGinty, Caroline S. Hill   +11 more
doaj   +1 more source

Spatial Light Modulator Microscopy [PDF]

Cold Spring Harbor Protocols, 2013
The use of spatial light modulators (SLMs) for two-photon laser microscopy is described. SLM phase modulation can be used to generate nearly any spatiotemporal pattern of light, enabling simultaneous illumination of any number of selected regions of interest.
Volodymyr, Nikolenko, Darcy S, Peterka, Roberto, Araya, Alan, Woodruff, Rafael, Yuste   +4 more
openaire   +2 more sources

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

Chlamydia-containing spheres are a novel and predominant form of egress by the pathogen Chlamydia psittaci

mBio
The egress of intracellular bacteria from host cells and cellular tissues is a critical process during the infection cycle. This process is essential for bacteria to spread inside the host and can influence the outcome of an infection.
Jana Scholz, Gudrun Holland, Michael Laue, Sebastian Banhart, Dagmar Heuer   +4 more
doaj   +1 more source

Comparative performance of airyscan and structured illumination superresolution microscopy in the study of the surface texture and 3D shape of pollen

, 2018
The visualization of taxonomically diagnostic features of individual pollen grains can be a challenge for many ecologically and phylogenetically important pollen types.
Allen, Andersen, Anderson, Chaturvedi, Dan, Faegri, Gustafsson, Gustafsson, Habuchi, Han, Heintzmann, Hesse, Holt, Huang, Jost, Kasuboski, Leung, Long, Lukosz, Mander, Mander, Mander, Muller, Nelson, Page, Peltre, Punt, Punyasena, Reimer, Sheppard, Sivaguru, Sivaguru, Sun, Tcheng, Traverse, Urban, Urban, Urban, Weiss   +38 more
core   +1 more source

LDAcoop: Integrating non‐linear population dynamics into the analysis of clonogenic growth in vitro

Molecular Oncology, EarlyView.
Limiting dilution assays (LDAs) quantify clonogenic growth by seeding serial dilutions of cells and scoring wells for colony formation. The fraction of negative wells is plotted against cells seeded and analyzed using the non‐linear modeling of LDAcoop.
Nikko Brix, Daniel Samaga, Katharina Gehr, Benedek Dankó, Mohamed Schumann, Guido Drexler, Ahmed Alnatsha, Georg Beyer, Ujjwal Mahajan, Martin Selmansberger, Julia Mayerle, Claus Belka, Horst Zitzelsberger, Kirsten Lauber   +13 more
wiley   +1 more source

Single shot three-dimensional imaging of dilute atomic clouds [PDF]

, 2014
Light field microscopy methods together with three dimensional (3D) deconvolution can be used to obtain single shot 3D images of atomic clouds. We demonstrate the method using a test setup which extracts three dimensional images from a fluorescent $^{87}$
Kasevich, Mark, Sakmann, Kaspar
core   +2 more sources

Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin

Molecular Oncology, EarlyView.
The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla, Jan Kosla, Magdalena Prechova, Lukas Frick, Patricia Bortel, Yasmin Borutzki, Andrea Bileck, Christopher Gerner, Samuel M. Meier‐Menches, Selma Osmanagic‐Myers, Martin Gregor   +10 more
wiley   +1 more source

Calibration approaches for fluorescence lifetime applications using time-domain measurements

Methods in Microscopy
This tutorial focuses on presenting experimental protocols for acquiring instrument response functions (IRF) and for calibrating the instruments using reference dyes with validated lifetime in time-domain fluorescence lifetime measurements.
Margineanu Anca
doaj   +1 more source