Results 61 to 70 of about 904,307 (267)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

Review of Detection Limits for Various Techniques for Bacterial Detection in Food Samples

open access: yesNanomaterials
Foodborne illnesses can be infectious and dangerous, and most of them are caused by bacteria. Some common food-related bacteria species exist widely in nature and pose a serious threat to both humans and animals; they can cause poisoning, diseases ...
Xinyi Zhao   +5 more
doaj   +1 more source

Enhancement of Characteristics of Field Asymmetric Ion Mobility Spectrometer with Laser Ionization for Detection of Explosives in Vapor Phase

open access: yesChemosensors, 2020
Ion mobility spectrometry instrumentation today is widespread in the area of transport security and counterterrorism. This method of detection of explosive substances is highly appreciated for the existence of portable detectors capable of detecting ...
Vitalii A. Kostarev   +3 more
doaj   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

open access: yesFEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes   +3 more
wiley   +1 more source

Development and Validation of a Suite of E-Probes for Electronic Diagnostic Nucleic Acid Analysis (EDNA) for 20 Graft-Transmissible Pathogens of Citrus Using MiFi and Blind Ring Testing Among Novice Users

open access: yesPhytoFrontiers
Global citriculture faces significant challenges from graft-transmissible pathogens, which threaten both productivity and biosecurity. To address these challenges, citrus quarantine germplasm programs worldwide are responsible for producing and ...
D. M. Nascimento   +10 more
doaj   +1 more source

Analytical Performance Evaluation of Three Commercial Rapid Nucleic Acid Assays for SARS-CoV-2

open access: yesInfection and Drug Resistance, 2021
Jie Yi, Xiao Han, Ziyi Wang, Yu Chen, Yingchun Xu, Jie Wu Department of Laboratory Medicine, and Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Peking Union Medical College Hospital, Chinese Academy of
Yi J, Han X, Wang Z, Chen Y, Xu Y, Wu J
doaj  

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

open access: yesFEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe   +3 more
wiley   +1 more source

Development and Validation of Novel HPLC Methods for Quantitative Determination of Vitamin D3 in Tablet Dosage Form

open access: yesPharmaceuticals
In the present work, an efficient isocratic HPLC method was developed for the precise and accurate estimation of vitamin D3 in tablet form. The chromatographic conditions comprised an L3 silica column (5 µm in particle size, 4.6 mm × 250 mm) with a ...
Muhammad Saqib Gohar   +5 more
doaj   +1 more source

Semi-Automated Data Analysis for Ion-Selective Electrodes and Arrays Using the R Package ISEtools

open access: yesSensors, 2019
A new software package, ISEtools, is introduced for use within the popular open-source programming language R that allows Bayesian statistical data analysis techniques to be implemented in a straightforward manner.
Peter W. Dillingham   +3 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

open access: yesFEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li   +2 more
wiley   +1 more source

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