Results 241 to 250 of about 2,452,838 (337)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Determining the risk factors of malaria and anemia in children between 6 and 59 months using the joint generalized linear mixed model on the 2021 Nigeria Malaria Indicator Survey dataset. [PDF]

Front Public Health
Mhelembe T, Ramroop S, Habyarimana F.
europepmc   +1 more source

PowRPriori: Power Analysis via Data Simulation for (Generalized) Linear Mixed Effects Models [PDF]

Markus Grill
openalex   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Identifying Risk Indicators of Cardiovascular Disease in Fasa Cohort Study (FACS): An Application of Generalized Linear Mixed-Model Tree. [PDF]

Arch Iran Med
Asadi F, Homayounfar R, Farjam M, Mehrali Y, Masaebi F, Zayeri F.   +5 more
europepmc   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Assessing risk indicators of intimate partner femicide considering victim's coping strategies to violence: A dynamic multilevel linear mixed model based on genetic algorithms. [PDF]

Heliyon
Garcia-Vergara E, Fernández-Navarro F, Becerra-Alonso D, Almeda N.   +3 more
europepmc   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Using N2pc variability to probe functionality: Linear mixed modelling of trial EEG and behaviour

Clayton Hickey, Damiano Grignolio, Vinura Munasinghe, David Acunzo   +3 more
openalex   +1 more source