Results 211 to 220 of about 843,130 (317)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Spatial and Temporal Instability of Slightly-Curved Particle-Laden Shallow Mixing Layers

In the present paper we present linear and weakly nonlinear models for the analysis of stability of particle-laden slightly curved shallow mixing layers. The corresponding linear stability problem is solved using spatial stability analysis.
Eglīte, Irina, Koliškins, Andrejs
core  

Non-linear measures of movement variability in multiple sclerosis: a clinical narrative review of Lyapunov exponent and entropy applications in balance and gait. [PDF]

Front Neurol
Banakheiri T, Panisset MG, Galea MP, Cofré Lizama LE.   +3 more
europepmc   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Reconsidering the Role of Age in Tear Film Instability: Real-World Evidence From a Specialized Dry Eye Clinic. [PDF]

Clin Ophthalmol
Khan AZ, Steinkjer T, Fineide FA, Yazdani M, Badian RA, Utheim TP.   +5 more
europepmc   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Linear Stability of a Convective Flow in a Vertical Pipe

The linear stability of a convective flow in a vertical pipe generated by internal heat sources of constant volume density is analyzed in the present paper. The linear stability problem is solved for different values of the parameters of the problem.
Koliškins, Andrejs, Vaillancourt, Rémi
core  

Preserving Gadolinium Speciation in Environmental Waters: Establishing Hold Times and Storage Protocols for Reliable Analysis. [PDF]

ACS ES T Water
Ezzaldine A, Kanaththage M, Rubasinghege G, Frey B, Escobar GP, Coyte R.   +5 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Parametric resonance and chaos in a duffing-type oscillator with periodic inertia modulation. [PDF]

Sci Rep
El-Borhamy M, Nasef AA, Attia AF, Sobhy I.   +3 more
europepmc   +1 more source