Results 281 to 290 of about 3,440,234 (310)

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

open access: yesFEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo   +4 more
wiley   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

open access: yesFEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Comparative analysis of gene regulation in single cells using Compass. [PDF]

open access: yesCell Rep Methods
Wan C   +7 more
europepmc   +1 more source

High-intensity helical flow: a double-edged sword in coronary artery haemodynamics. [PDF]

open access: yesR Soc Open Sci
Shen C   +4 more
europepmc   +1 more source

Housing and Support Intervention and Mortality Among Homeless Adults With Mental Illnesses: A Secondary Analysis of a Randomized Clinical Trial.

open access: yesJAMA Netw Open
Lachaud J   +13 more
europepmc   +1 more source

Impact of the Exposome in Type 1 Diabetes: Protocol for a Scoping Review.

open access: yesJMIR Res Protoc
Rollet M   +4 more
europepmc   +1 more source

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