Results 41 to 50 of about 328,017 (264)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Regulation of high-temperature microstructure evolution and recrystallization by pre-strain accumulation in Al–Zn–Mg–Cu alloys

Journal of Materials Research and Technology
This study systematically investigates the hot deformation behavior of Al–Zn–Mg–Cu alloy through pre-deformation compression tests at different temperatures (room temperature, RT, and cryogenic temperature, CT) with varying pre-strain levels, combined ...
Dejin Wei, Guoqun Zhao, Luyi Han, Kunpeng Deng   +3 more
doaj   +1 more source

Electrocapillary Actuation of Liquid Metal in Microchannels

Micromachines, 2022
Controllable deformation of liquid metal by electrocapillary actuation (ECA) is empirically characterized in fluidic channels at the sub-millimeter-length scale.
Saige J. Dacuycuy, Wayne A. Shiroma, Aaron T. Ohta   +2 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Innovative crosslinking and foaming Strategies for Advancing biodegradable composite foams: Enhancing Foamability, Flexibility, and thermal insulation

Materials & Design
Ensuring thermal insulation stability under an extreme condition and optimizing energy utilization efficiency emerge as a pivotal concern. Bio-based poly(butylene adipate-co-terephthalate) (PBAT) foams, characterized by irreplaceable flexibility ...
Zhaozhi Wang, Guilong Wang, Zhaorui Xu, Jialong Chai, Guoqun Zhao   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Ultrasonic welding of thermoplastic composites with woven mesh energy directors: Enhanced strength and hygrothermal resistance

Journal of Materials Research and Technology
In ultrasonic welding of thermoplastic composites, energy directors (EDs) based on thermoplastic films often exhibit low heat generation efficiency and uneven temperature distribution at the welding interface, which negatively affect weld quality and ...
Dongsheng Yue, Guoqun Zhao, Jiaying Pan, Jiaming Liu, Dong Quan   +4 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Unraveling nano-scale interfacial structure and strengthening mechanism of Al-TiB

Journal of Magnesium and Alloys
Controlling the interfacial evolution mechanisms in Al-TiB 2 /Mg laminated composites is critical for optimizing their mechanical performances. This study employed multiscale characterizations integrating quasi-in-situ analysis, to systematically ...
Lihua Qian, Liang Chen, Yuhui Zhao, Jianwei Tang, Cunsheng Zhang, Haimin Ding, Guoqun Zhao   +6 more
doaj   +1 more source