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Quantitative Measurement of the Kinase Activity of Wildtype ALPK1 and Disease-Causing ALPK1 Mutants Using Cell-Free Radiometric Phosphorylation Assays. [PDF]
Bio ProtocSnelling T.
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Chemiluminescence in liquid scintillation counting
Analytical Biochemistry, 1970Abstract A new chemiluminescence reaction arising from the combination of trichloroacetic acid and bathophenanthroline during the liquid scintillation counting of the Fe 59 beta emission is described. This is compared to the previously reported chemiluminescence arising from a reaction between quarternary amines and peroxides.
P.R. O'Bar, A.H. Woods
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A new scintillator for liquid scintillation counting
The International Journal of Applied Radiation and Isotopes, 1967Abstract This report describes the results of investigations carried out with a new scintillator: 2-(4′-t-Butylphenyl)-5-(4″-biphenylyl)-1,3,4-oxdiazole (Butyl-PBD). Counting efficiencies and background counts of variously quenched toluene and dioxane systems containing Butyl-PBD have been compared with similar systems containing PPO and DM-POPOP as ...
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Liquid scintillation counting of chlorophyll
The International Journal of Applied Radiation and Isotopes, 1975Abstract A precise and reproducible method of liquid scintillation counting was worked out for measuring the radioactivity of 14 C-labelled chlorophyll a and chlorophyll b solutions without previous bleaching. The spurious count rate caused by luminescence of the scintillant-chlorophyll system is eliminated by using a suitable scintillant and by
B. Horičková +2 more
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Liquid scintillation counting of blood
The International Journal of Applied Radiation and Isotopes, 1965Abstract Several methods are compared for liquid scintillation counting of blood samples. The radioactive products are extracted from the blood either directly with the scintillation solvent system “Polyether 611” or with solvents such as dioxane or ethanol. The last-mentioned extracts are counted in a hyamine-Cl-touluene system. Blood contacted with
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Liquid scintillation counting of H2S35
The International Journal of Applied Radiation and Isotopes, 1961The cap of a Packard sample vial was modifiod to permit ready injection of the gas and yet prevent leakage. A series of such vials containing increasing volumes of Hsub 2/S/sup 35/ was countod after various periods to determine the linearity of the calibration curve (a measure of both quenching and loss of gas to the vapor space) and the change of the ...
W. ten Hove, B.E. Gordon, H.R. Lukens
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Liquid scintillation counting at ambient temperature
Analytical Biochemistry, 1966Abstract Ambient-temperature liquid scintillation counting of aqueous samples of up to 2 ml volume realized effciencies of over 80% for 14 C and 20% for 3 H in a dioxane-naphthalene scintillator. The use of external standardization for rapid determination of counting efficiency is presented together with some characteristics of a newly available ...
T.C. Hall, C.J. Weiser
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Liquid scintillation counting of porphyrins
The International Journal of Applied Radiation and Isotopes, 1985Abstract Carbon-14-labelled porphyrins may be counted by liquid scintillation up to a vial concentration of ca. 3 × 10−5 M (ca. 0.5 mg/vial), either directly or after initial sorption onto chromatography paper. A readily-constructed quench curve permits direct conversion of cpm to Bq and is pratical for 14C to ca. 35% efficiency.
Brenda J. Deakin +2 more
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1979
Carbon —14 and tritium are β− emitting radioisotopes with β− emissions of very low energy which are extremely difficult to detect with any form of window counter, due to self-absorption of the β− particles and their absorption within the counter window.
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Carbon —14 and tritium are β− emitting radioisotopes with β− emissions of very low energy which are extremely difficult to detect with any form of window counter, due to self-absorption of the β− particles and their absorption within the counter window.
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An economical system for liquid scintillation counting
Analytical Biochemistry, 1974Abstract Small glass shell vials (12 × 35 mm minivials), containing 2.0 ml of a dioxane-based scintillation solution plus a 14 C-labeled sample, were placed in a conventional glass, 20-ml count vial and assayed in a scintillation spectrometer. Statistical comparison of counts recorded from 14 C samples prepared both in the minivial system and ...
R.W. Bovey, J.R. Baur
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