Results 211 to 220 of about 3,194,366 (258)

Effects of Dietary Mannan Oligosaccharides on Growth, Disease Resistance, and Gut Microbiota in Zebrafish

open access: yesAnimal Research and One Health, EarlyView.
Supplementation with mannan oligosaccharides (MOS) enhances growth, disease resistance, antioxidant capacity, and gut microbiota diversity while suppressing inflammation, apoptosis, and strengthening intestinal barrier integrity in zebrafish challenged with LPS or DSS.
Zhiyuan Lu   +4 more
wiley   +1 more source

Cross‐Species Single‐Cell Comparative Analysis Reveals Gluconeogenic Features of Hepatocytes in Ruminants

open access: yesAnimal Research and One Health, EarlyView.
In this study, we used cross‐species comparisons to identify ruminant hepatocyte features. We also identified potential genes and TFs involved in coordinating gluconeogenesis in ruminant hepatocytes. By integrating single‐cell data with population genetic analyses, we further identified candidate genetic variation in glucose metabolism and ...
Yaqi Zhou   +6 more
wiley   +1 more source

MiniSdd7‐Derived Cytosine Base Editor Promotes Efficient Multiplex Gene Knockout in Porcine Primary Fibroblasts

open access: yesAnimal Research and One Health, EarlyView.
The miniSdd7‐BE4max‐SpG system enables highly efficient multiplex C‐to‐T base editing of APN, CD163, and MSTN in porcine fibroblasts, providing a robust platform for future development of multiplex gene‐edited livestock. ABSTRACT Efficient multiplex genome editing is essential for improving complex traits in livestock that are governed by multiple ...
Shaoshuai Wang   +4 more
wiley   +1 more source

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

open access: yesAnimal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

Cumulative Reproductive‐Lactation Burdens Induce a Systemic Immunometabolic Trade‐Off Between Milk Yield and Mammary Health of Dairy Cows

open access: yesAnimal Research and One Health, EarlyView.
A population‐scale longitudinal transcriptomic atlas reveals how lactation stage and parity reshape systemic gene regulation in dairy cows. Multiomics‐inspired analyses uncover dynamic immune and metabolic adaptations that contribute to reproductive longevity and sustained productivity across the bovine lifespan.
Yangyang Shen   +8 more
wiley   +1 more source

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