Results 211 to 220 of about 2,732,997 (292)

Preliminary User-Centred Evaluation of a Bio-Cooperative Robotic Platform for Cognitive Rehabilitation in Parkinson's Disease and Mild Cognitive Impairment: Insights from a Focus Group and Living Lab in the OPERA Project. [PDF]

J Clin Med
Crocetto Y, Abagnale S, Martinelli G, Della Bella S, Pavan E, Rondoni C, Voscarelli A, Pirini M, Scotto di Luzio F, Zollo L, Cicarelli G, Polito C, Estraneo A.   +12 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Rationale and study protocol of the MAMELI Cohort study (Mapping the Methylation of repetitive elements to track the Exposome effects on health: the city of Legnano as a Living lab). [PDF]

PLoS One
Bollati V, Rota F, Dioni L, Favero C, Iodice S, Gallazzi M, Spinazzè A, Fanti G, Campagnolo D, Nardi T, Biganzoli D, Dariol E, Matsagani R, Hoxha M, Monti P, Albetti B, Tarantini L, Barbuto D, Luconi E, Marano G, Biganzoli G, Biciuffi R, Boracchi P, Cavallo DM, Guerrieri PM, Mosca F, Gustincich S, Sanesi G, Ferrari L, Carugno M, Fustinoni S, Pesatori AC, Pandolfini L, Miragoli M, Cattaneo A, Biganzoli E.   +35 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

A Fast-Track Training Program for Living Lab Methodologies: program components, acceptability, and participant perceptions. [PDF]

Open Res Eur
Helsen K, Van der Auwera V, Drijkoningen F, Petsani D, Santonen T, Bamidis P, Konstantinidis E, De Witte NAJ.   +7 more
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Living Lab Dementia: process evaluation of an academic-practice partnership in German long term care for people living with dementia - study protocol. [PDF]

BMC Geriatr
Leinen A, Bühler F, Köpke S, Löbberding M, Meyer G, Schneider-Schelte H, Wilm S, Bieber A, Dichter MN.   +8 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source