Results 131 to 140 of about 509,208 (268)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Assessment of Changes in Postural Stability and Load Distribution in Patients With Multiple Sclerosis After 12 Weeks of Self-Paced Exercises Programmed by a Physiotherapist: A Prospective Cohort Study. [PDF]

Med Sci Monit
Pawik M, Pawik Ł, Fink-Lwow F.
europepmc   +1 more source

Subcutaneous Fat Thickness Remarkably Influences Contact Pressure and Load Distribution of Buttock in Seated Posture. [PDF]

J Healthc Eng, 2021
Wang K, Chen Y, Huang S, Wang L, Niu W.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

No significant side-to-side differences in retropatellar load distribution using more than 7.5 years after isolated MPFL reconstruction: A CT-osteoabsorptiometry pilot study in nine patients. [PDF]

J Exp Orthop
Kampik L, Schneider F, Hofner JM, Arora R, Pallua JD, Runer A.   +5 more
europepmc   +1 more source

Contribution of sex, sports and activity types and curriculum load distribution to intracurricular injury risk in physical education teacher education: a cohort study. [PDF]

BMJ Open Sport Exerc Med, 2022
Barendrecht M, Tak I, Barten C, Verhagen E.   +3 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Training Load Distribution Across Weekly Microcycles According to the Match Schedule During the Regular Season in a Professional Rink Hockey Team. [PDF]

J Funct Morphol Kinesiol
Fortunati M, Drid P, Baptista R, Febbi M, Quintiero V, D'Antona G, Crisafulli O.   +6 more
europepmc   +1 more source

Dynamic Knee Joint Line Orientation Is Not Predictive of Tibio-Femoral Load Distribution During Walking. [PDF]

Front Bioeng Biotechnol, 2021
Trepczynski A, Moewis P, Damm P, Schütz P, Dymke J, Hommel H, Taylor WR, Duda GN.   +7 more
europepmc   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source