Results 101 to 110 of about 10,289,866 (260)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

open access: yesFEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez   +6 more
wiley   +1 more source

Creative small settlements. Culture-based solutions for local sustainable development. [PDF]

open access: yes, 2017
Culture can play a fundamental role in fostering sustainable patterns of urban and regional development. This is the message of the Global Report ‘Culture for Sustainable Urban Development’, which UNESCO has coordinated for the UN-HABITAT III Conference (
Verdini, G., Ceccarelli, P.
core  

Features of a Local Culture as Viewed from the Perspective of Strangers

open access: yesForum: Qualitative Social Research, 2012
This article describes a unique mixing of grounded theory methodology (GTM) and autoethnographic methods in a study of the difficulties faced by individuals (of the professional class) moving from the cosmopolitan urban areas of Mexico to a middle-sized,
Silvia Bénard
doaj  

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

open access: yesFEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton   +6 more
wiley   +1 more source

2017-18 Institute for Culture and Society Annual Review

open access: yes, 2018
The Institute for Culture and Society researches transformations in culture and society in the context of contemporary global change. It champions collaborative engaged research in the humanities and social sciences for a globalising digital age.
Institute for Culture and Society (R)   +1 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Risk culture in financial organisations [PDF]

open access: yes, 2013
Interest in the cultures of organisations and their effects on management practices goes back many years and there is an extensive body of scholarship on this topic. Yet this interest has increased dramatically in the period since 2008. The debate is led
Power, Michael   +2 more
core  

Big Local Partnership Reviews, 2015-2023

open access: yes
copyright UK Data Service and data collection copyright owner.Big Local is a long-term, place-based funding programme in England aimed at empowering residents in selected communities to lead local change, build community capacity, and improve outcomes ...
Local Trust
core   +1 more source

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