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Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne
2019Transient expression of inserted recombinant DNA in plant protoplasts is a widely used tool for functional genomics research. Recently it has been utilized to screen potential sgRNA guides for CRISPR-mediated genome editing. However, little research has been conducted into the use of transient expression of protoplasts in Lolium perenne (a globally ...
Anne Louise Maddison +3 more
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Synaptonemal complex formation in hybrids of Lolium temulentum � Lolium perenne (L.)
Chromosoma, 1985The chromosomes of Lolium temulentum are longer and contain on average 50% more nuclear DNA than the chromosomes of L. perenne. In the hybrid, despite the difference in length and DNA content, pairing between the homoeologous chromosomes at pachytene is effective and the chiasma frequency at first metaphase in pollen mother cells is high, about 1.6 per
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