Transposable elements are a serious threat for genome integrity and their control via small RNA mediated silencing pathways is an ancient strategy. The fruit fly Drosophila melanogaster has two silencing activities that target transposons: endogenous siRNAs (esiRNAs or endo-siRNAs) and Piwi-interacting small RNAs (piRNAs). The biogenesis of endo-siRNAs
Mirkovic-Hösle, Milijana +1 more
openaire +5 more sources
Quantification of Dehydroepiandrosterone, 17β-Estradiol, Testosterone, and Their Sulfates in Mouse Tissues by LC-MS/MS. [PDF]
We report a high-performance, liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) assay to quantify without derivatizaton dehyroepiandrosterone (DHEA), 17β-estradiol (E2), testosterone (T), and their sulfates in serum and tissues.
Napoli, Joseph L, Yoo, Hong Sik
core
A uracil nitroso amine based colorimetric sensor for the detection of Cu²⁺ ions from aqueous environment and its practical applications [PDF]
A simple uracil nitroso amine based colorimetric chemosensor (UNA-1) has been synthesized and screened for its cation recognition ability. Sensor UNA-1 exhibited a high sensitivity and selectivity towards Cu²⁺ ions in aqueous medium in the presence of a ...
Chen, Lingxin +10 more
core +2 more sources
Background. One of the important source of food contamination with elements harmful to human health such as lead or cadmium is migration from the articles intended to come into contact with food.
Monika Mania +3 more
doaj +1 more source
An all-glass microfluidic network with integrated amorphous silicon photosensors for on-chip monitoring of enzymatic biochemical assay [PDF]
A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding.
Caputo, Domenico +9 more
core +5 more sources
Determination of the LOQ in real-time PCR by receiver operating characteristic curve analysis: application to qPCR assays for Fusarium verticillioides and F. proliferatum [PDF]
Real-time PCR (qPCR) is the principal technique for the quantification of pathogen biomass in host tissue, yet no generic methods exist for the determination of the limit of quantification (LOQ) and the limit of detection (LOD) in qPCR. We suggest using the Youden index in the context of the receiver operating characteristic (ROC) curve analysis for ...
Nutz, Sabine +2 more
openaire +3 more sources
Associated varieties of modules over Kac-Moody algebras and $C_2$-cofiniteness of W-algebras
First, we establish the relation between the associated varieties of modules over Kac-Moody algebras \hat{g} and those over affine W-algebras.
Kac, T. Arakawa
core +1 more source
Enzymatic digestion and selective quantification of underivatised [delta]9-tetrahydrocannabinol and cocaine in human hair using gas chromatography-mass spectrometry. [PDF]
Gas chromatography-mass spectrometric (GC-MS) methods for drug analysis routinely employ derivatising reagents. The aim of this paper was to develop a method for the analysis of two recreational drugs, delta-9-tetrahydrocannabinol ([delta](9)-THC) and ...
Barker, James +3 more
core +3 more sources
Abstract The issues surrounding micro- and nanoplastics (MPs and NPs) are gaining importance as the knowledge about their distribution and impacts on the environment and human health grows. In order to gain a better understanding about the occurrence of those plastic particles and the pollution of different freshwater systems, the project ...
Juri Jander +10 more
openaire +1 more source
Impact of carwash discharge on stormwater quality (Toulouse, France) [PDF]
The contribution of discharge from carwashes to pollutant levels in stormwater was evaluated. Five carwashes and two outlets in the city of Toulouse (France) were selected. Water samples were collected from December 2006 to December 2007.
Sablayrolles, Caroline +3 more
core +2 more sources

