Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Enhanced Marginal Sensitivity Model and Bounds. [PDF]
J Am Stat AssocZhang Y, Xu W, Tan Z.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Seamless Phase I-II Cancer Clinical Trials Using Kernel-Based Covariate Similarity. [PDF]
Pharm StatMakino K, Makigusa N, Kojima M.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
On the minimum number of non-monochromatic simplices for Sperner labelings of a regular triangulation. [PDF]
PLoS OneCalvo Pascual LÁ +4 more
europepmc +1 more source
Searching in Euclidean Spaces with Predictions. [PDF]
Theory Comput SystCabello S, Giannopoulos P.
europepmc +1 more source
Estimating Mutual Information and Pearson Correlation on Neural Evoked Responses. [PDF]
NeuroinformaticsHukari A, Cotroneo SF, Salmelin R.
europepmc +1 more source
Structural Characterization of Calcium-Dependent Calmodulin-Calmidazolium Binding Using Capillary Vibrating Sharp-Edge Spray Ionization-Based Native Mass Spectrometry and In-Droplet Hydrogen-Deuterium Exchange Mass Spectrometry. [PDF]
ACS OmegaAhmed S +4 more
europepmc +1 more source