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Establishment of Rat Lymphatic Endothelial Cell Line
Microcirculation, 2003The objective of the present study was to establish a rat lymphatic endothelial cell line and then to investigate the morphological and immunohistochemical properties of the cells.The lymphatic endothelial cells of rat thoracic ducts were isolated enzymatically by trypsin digestion and were cultured in endothelium growth medium (EGM)-2 in an atmosphere
Risuke, Mizuno +4 more
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Gene expression profile of lymphatic endothelial cells
Cell Biology International, 2011The lymphatic system was first described at around the same time as the blood circulation centuries ago, but the biological function elucidation of LECs (lymphatic endothelial cells) is far less than that of BVECs (blood vascular endothelial cells). Since the discovery of molecular markers for LECs and exploration of lymphatic role in tumour metastasis,
Peiliang, Wang, Yongbo, Cheng
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Lymphatic endothelial cells actively regulate prostate cancer cell invasion
NMR in Biomedicine, 2016Lymphatic vessels serve as the primary route for metastatic spread to lymph nodes. However, it is not clear how interactions between cancer cells and lymphatic endothelial cells (LECs), especially within hypoxic microenvironments, affect the invasion of cancer cells.
Tariq, Shah +4 more
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Moving lymphatic endothelial cells about
Science, 2017Vascular Biology Lymphatic vessels return fluid and immune cells from peripheral tissues back to the circulation. The growth of new lymphatic vessels and their remodeling are critical for clearing infection and, less helpfully, for metastasis of many cancer subtypes. Williams et al.
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Harvesting and cryopreservation of lymphatic endothelial cells for lymphatic tissue engineering
Cryobiology, 2010In order to provide a suitable source of cells for lymphatic tissue engineering, the present study was designed to investigate techniques for harvesting and cryopreservation of human dermal lymphatic endothelial cells (LECs) in vitro. The LECs were isolated from children's foreskins and then cultured in endothelial growth medium-2 MV (EGM-2-MV) with 5%
Zhaohua, Jiang +3 more
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Mechanotransduction in lymphatic endothelial cells.
Lymphology, 2007Initial lymphatic vessel endothelial cells are connected to the surrounding elastic fibers by fibrillin anchoring filaments that have been hypothesized to favor interstitial fluid drainage in edema pulling apart interendothelial junctions. We hypothesized a biochemical mechanism involving mechanotransduction. This study was designed to verify whether a
ROSSI, A. +5 more
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Lymphatic Endothelial Cells, Lymphangiogenesis, and Extracellular Matrix
Lymphatic Research and Biology, 2006Exciting studies involving the molecular regulation of lymphangiogenesis in lymphatic-associated disorders (e.g., wound healing, lymphedema and tumor metastasis) have focused renewed attention on the intrinsic relationship between lymphatic endothelial cells (LECs) and extracellular matrix (ECM) microenvironment.
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Histochemical analysis of lymphatic endothelial cells in lymphostasis
Microscopy Research and Technique, 2001AbstractThe ultrastructure of endothelial cells of intestinal lymphatics and the thoracic duct (TD) and the relation to lymphostasis were examined in rats and monkeys. Localization of 5′‐nucleotidase (5′‐Nase) and endothelial nitric oxide synthase (eNOS) was studied.
R C, Ji, S, Kato
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Multiparameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells
2022Multiparameter fluorescence-activated cell sorting (FACS) procedure separates target cells from a total population of cells by using specific signatures that the target cell expresses on their cell surface. For human lymphatic endothelial cells (LECs) this relates to cell surface expression of the CD34LowCD31HighVEGFR-3HighPodoplaninHigh profile that ...
Theresa, Connor +2 more
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Transcriptional Control of Lymphatic Endothelial Cell Type Specification
2013The lymphatic vasculature is the "sewer system" of our body as it plays an important role in transporting tissue fluids and extravasated plasma proteins back to the blood circulation and absorbs lipids from the intestinal tract. Malfunction of the lymphatic vasculature can result in lymphedema and obesity. The lymphatic system is also important for the
Ying, Yang, Guillermo, Oliver
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