Directed Evolution of T7 RNA Polymerase Minimizes dsRNA By-product and Enables High-Fidelity mRNA Synthesis for Demanding Therapeutic Applications [PDF]
ResearchT7 RNA polymerase (T7 RNAP) is the most widely used enzyme for synthesizing therapeutic mRNA. However, RNA transcribed by T7 RNAP often contains double-stranded RNA (dsRNA) by-products that trigger innate immune responses and complicate purification ...
Weitong Qin +6 more
doaj +2 more sources
Hepatocyte apoptosis fragment product cytokeratin-18 M30 level and non-alcoholic steatohepatitis risk diagnosis: an international registry study [PDF]
Chinese Medical Journal, 2023 . Background:. Liver biopsy for the diagnosis of non-alcoholic steatohepatitis (NASH) is limited by its inherent invasiveness and possible sampling errors.
Huai Zhang +24 more
doaj +3 more sources
Serum Cytokeratin 18 M30 Levels in Chronic Hepatitis B Reflect Both Phase and Histological Activities of Disease [PDF]
Mediators of Inflammation, 2017 Chronic hepatitis B has highly a dynamic course with significant fluctuations of HBV-DNA and ALT impeding assessment of disease activity. New biomarkers of inflammatory versus noninflammatory stages of HBV infection are urgently needed.
Magdalena Świderska +6 more
doaj +2 more sources
Clinical Evaluation of M30 and M65 ELISA Cell Death Assays as Circulating Biomarkers in a Drug-Sensitive Tumor, Testicular Cancer [PDF]
Neoplasia: An International Journal for Oncology Research, 2008 Circulating full-length and caspase-cleaved cytokeratin 18 (CK18) are considered biomarkers of chemotherapy-induced cell death measured using a combination of the M30 and M65 ELISAs.
Esther C. de Haas +10 more
doaj +3 more sources
Corrosion resistance studies on the concrete made with basalt fibres and basalt fibre reinforced rebars [PDF]
E3S Web of Conferences, 2021 In the present work, assessment of corrosion resistance for M30 grade concrete made with basalt fibres in terms of Resistivity, Potentials and Chloride ion diffusion is made to understand the corrosion resistance studies of concrete specimen made with ...
Goud E Giri Prasad +3 more
doaj +1 more source
Food safety knowledge and practices among fresh coconut vendors
Scientific African, 2020 The study examines the distribution and sale practices of coconut vendors, identifies and analyzes food safety bottlenecks associated with street vending of fresh coconuts in Accra, Ghana.
C. Oduro-Yeboah +4 more
doaj +1 more source
Prognostic biomarkers in lung cancer patients in terms of long-term survival
Turkish Journal of Internal Medicine, 2021 Introduction: We aimed to investigate the predictive effect of serum M30 and M65 antigens on long-term prognosis in patients with advanced stage lung cancer before and after the first dose of chemotherapy.
Belkis Nihan Coskun +4 more
doaj +1 more source
Motivations of guests contributing sWOM on social media: a case in Vietnam [PDF]
Journal of Asian Business and Economic Studies, 2022 Purpose – This paper examines whether guests contribute sWOM (social word of mouth) on different SNSs (social networking sites) regarding various personal motivations. SNSs have changed the way guests eat and experience their food and dishes.
Le Thi Thanh Ha, Vo Thanh Thu
doaj +1 more source
Macrophage‐derived MLKL in alcohol‐associated liver disease: Regulation of phagocytosis
Hepatology, EarlyView., 2022 EtOH causes leaky gut allowing bacteria and PAMPs into the liver, resulting in hepatic inflammation and injury. We demonstrate that LPS induces STAT1‐mediated expression and phosphorylation of MLKL in macrophages and identify a novel function that myeloid MLKL translocates to phagosomes and lysosomes and regulates phagocytosis, which contributes to the
Xiaoqin Wu +16 more
wiley +1 more source
Journal of Fungi, 2020 The first characterized antifungal in the orotomide class is olorofim. It targets the de novo pyrimidine biosynthesis pathway by inhibiting dihydroorotate dehydrogenase (DHODH).
Saskia du Pré +7 more
doaj +1 more source