We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Expanding perspectives on specialized metabolism in Streptomyces bacteria. [PDF]
Tiller LA +3 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Socioeconomic Status, Alcohol Use, and Mortality: Results From a Prospective Cohort Study. [PDF]
Butovetsky I +6 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Socioeconomic factors, error positivity, and performance monitoring in children. [PDF]
Strack J +5 more
europepmc +1 more source
The Kia Tīmata Pai Randomized Controlled Trial: ENRICH Early Childhood Teacher Training Improves Toddlers' Oral Language and Self-Regulation. [PDF]
Reese E +17 more
europepmc +1 more source
Genome-Wide Association Analysis of Gibberellin Sensitivity for Panicle Exsertion Length in Rice and Candidate Gene Identification. [PDF]
Li Y +8 more
europepmc +1 more source
Neuropsychological Predictors of Treatment Dropout Among Intimate Partner Violence Perpetrators. [PDF]
Romero-Martínez Á +3 more
europepmc +1 more source

