Results 251 to 260 of about 15,385,090 (308)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Socioeconomic Status, Alcohol Use, and Mortality: Results From a Prospective Cohort Study. [PDF]

open access: yesAlcohol Clin Exp Res (Hoboken)
Butovetsky I   +6 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

open access: yesFEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima   +7 more
wiley   +1 more source

Socioeconomic factors, error positivity, and performance monitoring in children. [PDF]

open access: yesDev Cogn Neurosci
Strack J   +5 more
europepmc   +1 more source

The Kia Tīmata Pai Randomized Controlled Trial: ENRICH Early Childhood Teacher Training Improves Toddlers' Oral Language and Self-Regulation. [PDF]

open access: yesDev Sci
Reese E   +17 more
europepmc   +1 more source

Neuropsychological Predictors of Treatment Dropout Among Intimate Partner Violence Perpetrators. [PDF]

open access: yesJ Intell
Romero-Martínez Á   +3 more
europepmc   +1 more source

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