Results 21 to 30 of about 42,853 (110)

A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch–Agarose Mixture and Its Application for Convenient Protein–Protein Interaction Analysis

Methods and Protocols, 2023
The detection and quantification of protein–protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST).
Yuri Emoto, Ryoya Katayama, Emi Hibino, Sho Ishihara, Natsuko Goda, Takeshi Tenno, Yoshihiro Kobashigawa, Hiroshi Morioka, Hidekazu Hiroaki   +8 more
doaj   +1 more source

Accessing a hidden conformation of the maltose binding protein using accelerated molecular dynamics.

PLoS Computational Biology, 2011
Periplasmic binding proteins (PBPs) are a large family of molecular transporters that play a key role in nutrient uptake and chemotaxis in Gram-negative bacteria.
Denis Bucher, Barry J Grant, Phineus R Markwick, J Andrew McCammon   +3 more
doaj   +1 more source

The malQ gene is essential for starch metabolism in Streptococcus mutans

Journal of Oral Microbiology, 2013
Background: The malQ and glgP genes, respectively, annotated as putative 4-α-glucanotransferase and putative glycogen phosphorylase are located with a 29 nucleotide overlap on the Streptococcus mutans genome.
Yutaka Sato, Kazuko Okamoto-Shibayama, Toshifumi Azuma   +2 more
doaj   +1 more source

Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2

Bio-Protocol, 2015
The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E.
Konstantin Tomanov, Andreas Bachmair
doaj   +1 more source

Recombinant expression and purification of an Oxysterol Binding Protein from Aspergillus oryzae 3.042

BIO Web of Conferences, 2017
A full-length cDNA encoding a candidate Oxysterol-binding protein(OSBP) from Aspergillus oryzae (AoOSBP) was cloned and expressed in Escherichia coli as a maltose-binding protein (MBP) fusion protein.
Zhang Xian, Liu Lanlan, Hu Jianwen, Chen Fei, Zeng Bin   +4 more
doaj   +1 more source

Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS

BioTechniques, 2010
We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies.
Hu Tao, Wenjun Liu, Brandi N. Simmons, Helen K. Harris, Timothy C. Cox, Michael A. Massiah   +5 more
doaj   +1 more source

Expression, Purification, Refolding, and Characterization of a Neverland Protein From Caenorhabditis elegans

Frontiers in Bioengineering and Biotechnology, 2020
Steroid hormones that serve as vital compounds are necessary for the development and metabolism of a variety of organisms. The neverland (NVD) family genes encode the conserved Rieske-type oxygenases, which are accountable for the dehydrogenation during ...
Shuhong Mao, Shuhong Mao, Shuhong Mao, Shuhong Mao, Zhan Song, Mian Wu, Xiaorui Wang, Fuping Lu, Fuping Lu, Fuping Lu, Fuping Lu, Hui-Min Qin, Hui-Min Qin, Hui-Min Qin, Hui-Min Qin   +14 more
doaj   +1 more source

Protein-mediated miRNA detection and siRNA enrichment using p19

BioTechniques, 2010
p19 RNA binding protein from the Carnation Italian ringspot virus (CIRV) is an RNA-silencing suppressor that binds small interfering RNA (siRNA) with high affinity.
Jingmin Jin, Melissa Cid, Catherine B. Poole, Larry A. McReynolds   +3 more
doaj   +1 more source

Solution NMR studies of periplasmic binding proteins and their interaction partners

Biomolecular Concepts, 2011
Periplasmic binding proteins (PBPs) are a crucial part of ATP-binding cassette import systems in Gram-negative bacteria. Central to their function is the ability to undergo a large-scale conformational rearrangement from open-unliganded to closed ...
Pistolesi Sara, Tjandra Nico, Bermejo Guillermo A.   +2 more
doaj   +1 more source

Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process

Microbial Cell Factories, 2019
Background The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs).
Animesh Sarker, Abhishek Singh Rathore, Rinkoo Devi Gupta   +2 more
doaj   +1 more source