Computational analysis of maltose binding protein translocation [PDF]
Philosophical Magazine, 2011 We propose a computational model for the study of maltose binding protein translocation across alpha-hemolysin nanopores. The phenomenological approach simplifies both the pore and the polypeptide chain; however it retains the basic structural protein-like properties of the maltose binding protein by promoting the correct formation of its native key ...
M. Chinappi, F. Cecconi, C. Casciola
semanticscholar +6 more sources
Role of denaturation in maltose binding protein translocation dynamics. [PDF]
The Journal of Physical Chemistry B, 2012 We present a computational study on the driven transport of the Maltose Binding Protein (MBP) across nanochannels in the framework of coarse-grained modeling. The work is motivated by recent experiments on voltage-driven transport of MBP across nanopores
Marco Bacci +3 more
semanticscholar +8 more sources
In vitro recombination of non-homologous genes can result in gene fusions that confer a switching phenotype to cells. [PDF]
PLoS ONE, 2011 Regulation of protein activity is central to the complexity of life. The ability to regulate protein activity through exogenously added molecules has biotechnological/biomedical applications and offers tools for basic science.
Richard A Heins +3 more
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Prokaryotic soluble overexpression and purification of bioactive human growth hormone by fusion to thioredoxin, maltose binding protein, and protein disulfide isomerase. [PDF]
PLoS ONE, 2014 Human growth hormone (hGH) is synthesized by somatotroph cells of the anterior pituitary gland and induces cell proliferation and growth. This protein has been approved for the treatment of various conditions, including hGH deficiency, chronic renal ...
Minh Tan Nguyen +8 more
doaj +2 more sources
PLoS ONE, 2014 Human granulocyte colony-stimulating factor (hGCSF), a neutrophil-promoting cytokine, is an effective therapeutic agent for neutropenia patients who have undergone several cancer treatments. Efficient production of hGCSF using E.
Bich Hang Do +4 more
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Prokaryotic Soluble Overexpression and Purification of Human VEGF165 by Fusion to a Maltose Binding Protein Tag. [PDF]
PLoS ONE, 2016 Human vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis and plays a central role in the process of tumor growth and metastatic dissemination. Escherichia coli is one of the most common expression systems used for the production
Minh Tan Nguyen +13 more
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Correction: Prokaryotic soluble overexpression and purification of human VEGF165 by fusion to a maltose binding protein tag. [PDF]
PLoS ONE, 2017 [This corrects the article DOI: 10.1371/journal.pone.0156296.].
Minh Tan Nguyen +13 more
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Induced Fit or Conformational Selection? The Role of the Semi-closed State in the Maltose Binding Protein [PDF]
Biochemistry, 2011 A full characterization of the thermodynamic forces underlying ligand-associated conformational changes in proteins is essential for understanding and manipulating diverse biological processes, including transport, signaling, and enzymatic activity ...
Denis Bucher +2 more
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Electronic Journal of Biotechnology, 2015 Background: The fermentation conditions of recombinant maltose-binding protein fused to neutrophil-activating protein (rMBP-NAP) of Helicobacter pylori were optimized from Escherichia coli TB1 with varying medium, inoculum age and size, time, inducer, pH
Jike Lu +5 more
doaj +2 more sources
Generation and Validation of Monoclonal Antibodies Against the Maltose Binding Protein. [PDF]
Monoclon Antib Immunodiagn Immunother, 2016 The maltose binding protein (MBP) is a commonly used protein tag. Two monoclonal antibodies (mAbs) were generated against the MBP by immunizing mice with purified 6xHis-tagged MBP (6xHis-MBP). A nontoxic adjuvant cocktail of poly(I:C) and anti-CD40 mAb was used.
Park YN, Glover RA, Daniels KJ, Soll DR.
europepmc +5 more sources