Conformational Dynamics in the Binding-Protein-Independent Mutant of the Escherichia coli Maltose Transporter, MalG511, and Its Interaction with Maltose Binding Protein. [PDF]
Bajaj R +3 more
europepmc +3 more sources
Identification of a system required for the functional surface localization of sugar binding proteins with class III signal peptides in Sulfolobus solfataricus [PDF]
The hyperthermophilic archaeon Sulfolobus solfataricus contains an unusual large number of sugar binding proteins that are synthesized as precursors with a class III signal peptide.
Albers S.V. +12 more
core +32 more sources
Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag. [PDF]
Duong-Ly KC, Gabelli SB.
europepmc +2 more sources
Maltose-binding protein fusion allows for high level bacterial expression and purification of bioactive mammalian cytokine derivatives. [PDF]
Fusokines are chimeric proteins generated by the physical coupling of cytokines in a single polypeptide, resulting in proteins with highly pleiotropic activity and the potential to treat cancer and autoimmune ailments.
Andrea Pennati +2 more
doaj +2 more sources
Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein. [PDF]
Nguyen AN +13 more
europepmc +3 more sources
Both Ligands and Macromolecular Crowders Preferentially Bind to Closed Conformations of Maltose Binding Protein. [PDF]
Ghosh A +4 more
europepmc +3 more sources
Strep-tag II Mutant Maltose-binding Protein for Reagentless Fluorescence Sensing. [PDF]
Hasmoni SH +4 more
europepmc +2 more sources
Protein modification with non-canonical amino acids (ncAAs) represents a useful technology to afford homogenous samples of bioconjugates with site-specific modification.
Alexander C. Williard +10 more
doaj +1 more source
Backbone assignment of crystalline E. coli maltose binding protein [PDF]
The E.coli maltose binding protein (MBP) is a 42.5 kDa molecule widely employed in many biotechnology applications. Because of its molecular size, it has become the main model system for the development of solution NMR methods adapted to large biomolecular targets.
Schubeis, Tobias +2 more
openaire +2 more sources
Thermodynamics of maltose binding protein unfolding [PDF]
AbstractThe maltose binding protein (MBP or MalE) of Escherichia coli is the periplasmic component of the transport system for malto‐oligosaccharides. It is used widely as a carrier protein for the production of recombinant fusion proteins. The melting of recombinant MBP was studied by differential scanning and titration calorimetry and fluorescence ...
V, Novokhatny, K, Ingham
openaire +2 more sources

